2.4. Measurement of intracellular ROS production The ROS assay involved measuring the fluorescence flux of DCFH-DA in LPS-induced Caco-2 cells, as described elsewhere (Wolfe & Liu, 2007). In brief, after the culture medium was removed, DCFH-DA (20 µM) was added to the cells and incuba...
(2 g/kg body weight) for the measurement of plasma pyruvate levels using an enzymatic assay. One week after the PTT, the GTT was performed with a similar fasting period followed by an intraperitoneal injection of glucose (2 g/kg body weight). The ITT was conducted one week following the ...
Simplified spectrophotometric assay for microsomal 3-hydroxy-3-methylglutaryl CoA reductase by measurement of coenzyme A. J. Lipid Res. 1973, 14, 625–631. [Google Scholar] [PubMed] Rudack, D.; Chisholm, E.M.; Holten, D. Rat liver glucose 6-phosphate dehydrogenase. Regulation by ...
First, the −30-min and 0-min glucose and insulin samples were pooled to provide a single fasting measurement (0-min). Second, although separate analyses were conducted for fructose and allulose, the data for the two controls (0 g fructose and 0 g allulose) were pooled for all ...