subfamilies that are designated alphabetically in the order in which they are described (e.g., *2A, *2B, and *2C). Unlike many heritable disease mutations, each CYP2D6 allele may include several single-nucleotide polymorphisms — a haplotype, rather than a single-site mutation. The *1 (...
aLucy足够耐心地等待别人而不生气 Lucy waited for enough patiently others are not angry[translate] aMelanin contents were spectrometrically determined as described previously (Choi et al., 2007) except for the following modifications:five zebrafish larvae, at 72 hpf, were used per measurement, and ...
Al- though there are many limitations (described following), association studies are perhaps the best for mapping of polygenic complex disease loci. However, this type of study requires a large number of patients and an adequate control group to achieve over 80% power to detect a locus. Once ...
The procedure for total RNA extraction is described below. The amplified fragment was fused in-frame with the 5′ terminus of the open reading frame of synthetic GFP (sGFP), then the 5930-bp fragment of FLO2:sGFP was cloned into BamHI and NotI sites of the vector CYH8, which was a...
Quantitative assays to measure the binding of defined synthetic antigenic peptides and purified MHC class I molecules are described for several common human HLA-A alleles (A1, A2.1, A3, A11 and A24). Under appropriate conditions, the binding of radiolabeled peptides to purified MHC class I ...
What is an inheritance relationship in which neither of two alleles of the same gene totally mask the other called?Non-Mendelian InheritanceNon-Mendelian inheritance describes inheritance patterns of traits that do not segregate or follow the principle of domin...
The beads were then pelleted by centrifugation, the supernatant was saved as the NB-fraction, and then the beads were washed as described previously for the anti-FLAG M2-agarose beads. The B-fraction was eluted from the beads by adding 50 μl 3× SDS-PAGE sample loading buffer to the ...
Clonally repopulated animals were killed and cells from BM, spleen, thymus and lymph nodes (LNs) were analysed for the presence of terminally differentiated B cells, T cells and myeloid cells of donor origin, as well as progenitor donor cells (antibodies used are described in Supplementary Table...
Overall, the balance parasites must maintain between anthelmintic resistance and competitive fitness when drugs are not present is an extremely relevant approach to understand anthelmintic effectiveness. 4.4. Future avenues and methods of research The approach described here shows that hypotheses generated ...
(Matsui et al., Journal of Bacteriology 169 (11): 5330-5332 (1987)) or similar analogs. The genes or alleles encoding these desensibilized anthranilate synthases are also referred to as trpEFBRalleles. Examples of mutants or alleles of this kind are described, for example, in U.S. Pat....