Amplification was performed with biotinylated goat-anti-rabbit secondary antibodies (1:200; Vector Laboratories, Burlingame, CA, USA) and avidin-biotin complex (1:800; Vector Laboratories). Chromogen development was performed with diaminobenzidine. Photographs were collected using a Nikon E800u ...
This list — with many of the blurbs owing a debt to the wealth of theme song history in the bookTV’s Greatest Hitsby Jon Burlingame— is our attempt to explain why we chose these 100 over any or all of your favorites. Enjoy, and get to humming. ...