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(or as indicated in the figure legend) and then used for DNA extraction as described below. For generation of stable cell lines expressing Tandem and Tandem-HHR DdCBEs, Flp-In-3T3 mouse cells were transfected with 3,200 ng of the expression vector, together with 3,200 ng of the Flp...
(sgRNA) sequences targeting the region adjacent to the exon 3 ofmboat7were picked using the online CRISPR design tool from the Zhang lab at the Broad Institute. The sgRNA sequences, listed in Supplementary Fig.2b, were then cloned into the pSpCas9 (BB)-2A-Puro (PX459) vector (a gift...
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