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Thawing of frozen tissue prior to lysis may result in RNA degradation and loss of RNA yield. Additional Materials Needed: You will need the following additional items not provided with this unit: DEPC-treated water Chloroform (molecular biology ...
One of the most important applications of alkaline proteases is in detergent and sanitizers industries. The presence of endospores in Bacillus spp. is very ef- fective and efficient in isolation, passage, preserving and ultimately production of valuable biological products. In this research, the ...
Whole cell extracts were prepared with Laemmli Buffer (4% SDS, 20% glycerol, and 120 mm Tris-HCl (pH 6.8)) and sonicated followed by boiling at 100 °C. The samples were electrophoresed on 7.5, 11, or 15% SDS-PAGE and transferred on nitrocellulose membrane followed by blocking with non...
PKC isozymes regulate gene expression and a variety of cellular functions, including growth, differentiation, tumor promotion, aging, and apoptosis (1, 2, 5); however, the biological significance of the heterogeneity in the PKC family is not clear. The distinct subcellular distribution, the presenc...
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Keywords: forage quality; forage digestibility; protein content; neutral detergent fibre; acid detergent fibre; leaf/stem ratio 1. Introduction The nutritive value of forage harvested for feeding domestic herbivores is generally estimated by two characteristics: (i) the concentration in Crude Protein (...