鉴于m6A在调节靶RNA的功能和基因表达方面的重要作用,作者在GVAX/Anti-PD-1治疗的第12天,通过B16肿瘤的LC-MS/MS检测了Alkbh5对RNA中m6A含量的影响,显示仅Alkbh5敲除组的m6A水平显著升高。然后,作者进行了m6A RNA免疫沉淀和高通量测序(M...
关键词 N6-甲基腺嘌呤;A l k B同源物5;分子生物学功能;疾病 中图分类号 R394 表观遗传是指在D N A序列不发生改变的情况下,基因功能出现稳定可遗传的变异,如组蛋白修饰㊁D N A甲基化,R N A甲基化,染色质重塑等[1]㊂越来越多研究表明,m6A是R N A分子上广泛存在的一种表观遗传修饰,参与R N A...
Up to now, methyltransferase-like 3 (METTL3), methyltransferase-like 14 (METTL14), methyltransferase-like 16 (METTL16), Wilms tumor 1 associated protein (WTAP), zinc finger CCCH-type containing 13 (ZC3H13) proteins, RNA-binding motif protein 15 (RBM15), Vir-like m6 A methyltransferase-asso...
一些报告显示,HIFs激活了m6一种去甲基化酶ALKBH5 [42–44],我们假设肝脏的低氧条件会增加ALKBH5的表达,并影响HBV RNA的甲基化状态及其在感染细胞中的丰度。我们的研究确定了 m 的作用6在缺氧条件下,5'ε茎环在调节pC和pgRNA半衰期和剪接方面的修饰。此外,我们确定了 ALKBH5 在缺氧条件下调节 HIF-1α 的作用,...
The fat mass and obesity-associated protein (FTO), an m6Amand m6A mRNA demethylase, has been reported to affect cellular metabolism in acute myeloid leukemia (AML). ALKBH5, the specific RNA m6A demethylase, controls oncogene expression in AML. ALKBH5 becomes highly expressed in hematopoietic ...
et al. Single-nucleotide-resolution mapping of m6A and m6Am throughout the transcriptome. Nat Methods 12, 767–72 (2015). 14. Lee, M., Kim, B. & Kim, V. N. Emerging roles of RNA modification: m(6)A and U-tail. Cell 158, 980–7 (2014). 15. Wang, X. et al. N6-...
Surprisingly, according to an m6A RNA methylase target gene database prediction website-m6a2 target (http://m6a2target.canceromics.org/#/validation), we found that IRF3 is likely to bind to ALKBH5 and identified 12 potential binding sites via SRAMP (http://www.cuilab.cn/cramp) (Fig. 6A...
m6Am: N6,2-O-dimethyladenosine m1A: N1-methyladenosine m3T: 3-Methylthymine ssDNA: Single-stranded DNAs m3U: 3-Methyluracil ssRNA: Single-stranded RNA NRL: Nucleotide recognition lid BCSC: Breast cancer stem cell 3′UTR: 3′ Untranslated region G6PD: Glucose-6-phosphate dehydroge...
As ALKBH5 may be involved in NSCLC pathogenesis, we investigated potential m6A-modified targets of ALKBH5 in human NSCLC cells using m6A2Target (http://m6a2target.canceromics.org) and SRAMP. We focused on JAK2 because it has multiple high-confidence predicted m6A modification sites (Figure S1...
Cytoplasmic FTO inhibits cancer stem cell (CSC) capabilities in colorectal cancer via its m6Am demethylase activity [32]. Furthermore, it has been confirmed that the FTO protein possesses a nuclear localization signal (NLS), aiding its shuttling between the nucleus and the cytoplasm, thereby ...