Enzymatic activity that hydrolyzes cholinephosphate and p-nitrophenyl phosphate (alkaline phosphatase, EC 3.1.3.1) has been found in two strains of HeLa cells. Both phosphatase activities were primarily associated with the microsomal fraction and had pH optima of 9–9.5. Double reciprocal plots indicat...
英文名称: Alkaline Phosphatase,Calf Intestinal 纯度: 99 % 产地: 中国/上海 品牌: 泽叶生物 包装规格: 500U 货号: ZY120404 别名: 碱性磷酸酶,牛小肠 碱性磷酸酶,牛小肠(Alkaline Phosphatase,Calf Intestinal)产品及特点: Calf Intestinal Alkaline Phosphatase(CIAP)牛小肠碱性磷酸酶与E.coli来源的酶...
Changes in alkal ine phosphatase act ivity and phosphat e uptake in P-limited phytoplankton, induced by light intensity and spectral quality[ J] . Hydrobiol, 1998, 160: 173-178.Wynne D,Rhee G Y.Changes in alkaline phosphatase activity andphosphate uptake in P-limited phytoplankton,induced by ...
1活性单位指在37℃下, 1×Alkaline Phosphatase反应缓冲体系下,1分钟内水解对硝基苯磷酸盐 (p-nitrophenyl phosphate,pNPP) 生成1 μmol的对硝基苯酚 (p-nitrophenol) 所需要的酶量。 活性测定条件 活性定义反应液组成:50 mM Glycine–NaOH (pH 10.5),20 mM MgCl2,5mM ZnCl2,100 mM KCl,5mM pNPP。
Alkaline phosphatase (tissue-nonspecific isoenzyme) is a phosphoethanolamine and pyridoxal-5'-phosphate ectophosphatase: normal and hypophosphatasia fibrob... To clarify its physiologic role, alkaline phosphatase (ALP) was examined in normal skin fibroblasts and was shown to be the tissue-nonspecific (...
The phosphate regulation and subcellular location of the hydrolytic enzyme alkaline phosphatase were investigated in the Gram-negative bacterium Zymomonas mobilis. The biosynthesis of alkaline phosphatase was not derepressed at a low phosphate concentration as is generally observed in other micro-organisms,...
Alkaline Phosphatase, with the chemical formula unknown and CAS registry number 9001-78-9, is an enzyme that plays a crucial role in various biological processes. This enzyme is commonly found in tissues such as bone, liver, and intestine, where it catalyzes the hydrolysis of phosphate esters ...
Alkaline phosphatase, a cell surface membrane-boundenzymeattached to the cell membrane by means of a phosphatidyl-inositol-glycan anchor, catalyzes thehydrolysisof monophosphate esters at basic pH-releasing inorganic phosphate. There are fourisoenzymesofalkaline phosphatasedepending on their tissue site of...
The result suggests that increased enzyme activity and its induction during sporulation in parent strain has some functional linkage with sporulation rather than phosphate starvation. 展开 关键词: Alkaline phosphatase mutant sporulation Anabaena doliolum Abbreviation MNNG N-methyl-N T -nitro-N-...
To examine the effects of phosphine on phosphorus uptake and utilization in Thalassiosira pseudonana, we examined the transcriptional level of the phosphate transporter gene (TpPHO) and the activity of alkaline phosphatase (AKP) in relation to supplement of various concentrations of phosphine. TpPHO ...