SHP2 is an intracellular phosphatase involved in transducing RTK signals55,56 and co-therapies with SHP2 inhibitors can enhance response to ALK inhibitors57,58. Paralleling our results with EGFRi and MMPi, SHP2i co-treatment with ALKi suppressed RTK reactivation pulses and enhanced cell killing, ...
(50 mM Tris-HCl, 1% NP-40, 0.1% SDS, 0.1% Na-deoxycholate, 0.1 mM EDTA, 0.1 mM EGTA, 200 μM NaF, 20 μM Na-pyrophosphate, 2 mg ml−1 complete protease inhibitor, 0.3 mg ml−1 Pefabloc phosphatase inhibitor, 40 mM β-glycerophophate, 2 mM Na3...
Cells were lysed in RIPA buffer (#9806S, CST) with 1 mM PMSF (P7626, MilliporeSigma), and Phosphatase Inhibitor Cocktail 2 (P5726, MilliporeSigma). After quantifying total protein concentration with the BCA assay (23225, ThermoFisher), 10 μg of each sample was boiled at 95°C for 5 ...
In the previous section we discussed the many important activities influenced by AP-1 proteins in cHL and ALK+ ALCL. In this section we will examine the mechanisms and signalling events that lead to their elevated expression and/or activation in these lymphomas. Atsaves et al. reported thatJ...
RNA-RNA in situ hybridization using digoxigenin-labeled probes: the use of high-molecular-weight polyvinyl alcohol in the alkaline phosphatase indoxyl-nitroblue tetrazolium reaction Anal. Biochem., 215 (1993), pp. 86-89 View PDFView articleView in ScopusGoogle Scholar Foo et al., 2006 S.S. ...
After treatment, cells were incubated with DiOC6(3) and DAPI diluted in medium, for 30 min at 37 °C/5% CO2 [60, 61] and analyzed by means of a MACSQuant flow cytometer (Miltenyi Biotec, Bergisch Gladbach, Germany). Data were analyzed with FlowJo software (Ashland, Oregon, USA)....
Cells or minced xenografts were washed in cold PBS (Nacalai Tesque) then lysed on ice in a lysis buffer (50 mM Tris pH 6.8, 1% SDS, 1 mM EDTA, 10% glycerol, 1× Complete Mini Protease Inhibitor Cocktail [Roche Applied Science, Penzberg, Germany], and 1× PhosStop Phosphatase Inhi...