Compounds were purified using a Biotage Isolera One system by normal phase chromatography using Biotage SNAP KP-Sil or Sfär Silica D columns (part no. FSKO-1107/FSRD-0445) or by reverse-phase chromatography using Biotage SNAP KP-C18-HS or Sfär C18 D columns (part no. FSLO-1118/FS...
(six times, 5 min each) and mounted with 1% normal serum in PBS for 30 min. The primary antibody was subsequently applied at 4 °C overnight. Glass slides were then incubated with the secondary antibody for 40 min, followed by washes in PBS (six times, 5 min each). ...