在健康的大脑中,星形胶质细胞不具分裂特性,因此逆转录病毒感染的星形胶质细胞很少且不足以实现组织修复。因此,为追踪小鼠皮质中静息态的星形胶质细胞原位向神经元的转化过程,陈功教授等利用Aldh1l1-CreERT2与Ai14小鼠杂交建立的星形胶质细胞谱系追踪小鼠,利用腺相关病毒在谱系示踪的星形胶质细胞过表达NeuroD1,双光子连...
品系类型为BAC转基因，携带相关基因Aldh1l1，其主要特点是小鼠对绝大多数星形胶质细胞具有高水平的他莫昔芬诱导的Cre重组酶表达，而在神经元中则无显著表达。Aldh1l1-Cre/ERT2品系小鼠在体内可以进行泛星形胶质细胞、特异性和可诱导的基因操作，对神经回路/突触、行为、疾病和损伤/创伤的不同发育阶段的...
使用Aldh1l1-CreERT2 小鼠,科研工作者可特异性标记神经系统中星形胶质细胞,进而通过基因干扰、基因过表达等手段特异性探究单一脑区内胶质细胞及其内部某分子的功能,亦有潜力开展神经转分化相关基因治疗。 其中,CreERT2 系统需要腹腔注射他莫昔芬或 4-羟基-他莫昔芬来诱导 Cre 表达。星形胶质细胞在调节成年神经再生、...
使用Aldh1l1-CreERT2 小鼠,科研工作者可特异性标记神经系统中星形胶质细胞,进而通过基因干扰、基因过表达等手段特异性探究单一脑区内胶质细胞及其内部某分子的功能,亦有潜力开展神经转分化相关基因治疗。 其中,CreERT2 系统需要腹腔注射他莫昔芬或 4-羟基-他莫昔芬来诱导 Cre 表达。星形胶质细胞在调节成年神经再生、...
Objective: To characterize the cell specificity of Aldh1l1-cre/ERT2-driven recombination in the spleen and spinal cord of mice during peak experimental autoimmune encephalomyelitis (EAE).Background: Transgenic mouse models are deployed to study astrocyte-specific alterations in gene expression in in ...
将2A-CreERT2插入到小鼠Aldh1l1基因终止密码子处。 *使用本品系发表的文献需注明: Aldh1l1-2A-CreERT2 mice (Cat. NO. NM-KI-225010) were purchased from Shanghai Model Organisms Center, Inc.. 验证数据 Fig1. CreERT2-mediated recombination in brain of Aldh1l1CreERT2/+;Rosa26tdTomato/+mouse....
品系状态:精子冻存 | 目录号:NM-CKO-240585 Aldh1l1-2A-tdTomato-IRES-Cre 品系名:C57BL/6Smoc-Aldh1l1em1(2A-tdTomato-IRES-Cre)Smoc 品系状态:在研 | 目录号:NM-KI-242463 Aldh1l1-KO 品系名:C57BL/6Smoc-Aldh1l1em1Smoc 品系状态:精子冻存 | 目录号:NM-KO-226163你...
方法:首先运用CRISPR/Cas9系统构建了Aldh1a1-CRE小鼠,结合立体定位注射CRE重组酶依赖的腺相关病毒对Aldh1a1神经元进行特异标记与调控.通过神经环路顺行和逆行示踪技术,探究Aldh1a1神经元相关的神经环路.其次,运用CRISPR/Cas9系统构建了Aldh1a1-/--CRE小鼠,实现了对腹侧被盖区Aldh1a1神经元特异性标记调控的同时,实现...
(1μL in each hemisphere). Viruses used: AAV-GFAP-Cre-mCherry, lenti-pSico-Scr/shSIRT2/shALDH1A1-eGFP, were generated at the Institute for Basic Science Virus Facility (https://www.ibs.re.kr/virusfacility/). Mice were used 2–3 weeks after injection for behavior experiments and 6 ...
spontaneous IPSC amplitude and frequency recorded from these cells (Fig.5C middle, right). Since the targeted genes were knocked down only in astrocytes that successfully expressed the AAV-GFAP-Cre-mCh construct, the reduction in tonic GABA levels was not as absolute as observed in the in vitro...