Alcohol-forming fatty acyl reductases (FARs) catalyze the reduction of thioesters to alcohols and are key enzymes for microbial production of fatty alcohols. Many metabolic engineering strategies utilize FARs to produce fatty alcohols from intracellular acyl-CoA and acyl-ACP pools; however, enzyme act...
The present disclosure provides methods useful for producing fatty alcohol compositions from recombinant host cells. The disclosure further provides variant fatty acyl-CoA reductase (FAR) enzymes, polynucleotides encoding the variant FAR enzymes, and vectors and host cells comprising the same.Behnaz ...
Thus, there are at least two pathways existing among prokaryotes for the reduction of activated acyl substrates to fatty alcohol. The Marinobacter fatty acyl reductase studied has a wide substrate range in comparison to what can be found among enzymes so far studied in eukaryotes. Highlights ► ...
A more environment-friendly and sustainable route appears to be enzymatic conversion of microbial FA by fatty-acyl CoA reductase (FAR). These enzymes are either alcohol-forming or aldehyde-forming type; former class is identified mainly in plants, mammals, nematodes, while the latter is found in...
For instance, wax ester synthase/acyl-coenzyme A: diacylglycerol acyltransferase (WS/DGAT), a member of AATs family, utilizes fatty alcohols and fatty acyl-CoAs to synthesize the corresponding wax esters. WS/DGAT from the Marinobacter aquaeolei VT8 (Mal) have the most favored substrates with ...
(ADH) or aldehyde reductase (AHR) [1,90] or (B) two-step reduction of an activated FA by an alcohol-forming fatty acyl-CoA reductase (FAR) [16,43] with an aldehyde intermediate that is formed during the four-electron transfer but which is not released from the enzyme [32,85]. ...
Homologous antisense constructs were used to down-regulate tobacco cinnamyl-alcohol dehydrogenase (CAD; EC 1.1.1.195) and cinnamoyl-CoA reductase (CCR; EC ... Ralph,R D,Hatfield,... - 《Proceedings of the National Academy of Sciences of the United States of America》 被引量: 395发表: 1998...
Geranylgeranyl Pyrophosphate (ammonium salt)价格:￥1520/支产品详情:Geranylgeranyl pyrophosphate is an intermediate in the HMG-CoA reductase pathway derived directly from farnesyl pyrophos... 纯度:98% 包装规格:1 mg 用途:科学研究、研发 货号:CY15097 ...
A more environment-friendly and sustainable route appears to be enzymatic conversion of microbial FA by fatty-acyl CoA reductase (FAR). These enzymes are either alcohol-forming or aldehyde-forming type; former class is identified mainly in plants, mammals, nematodes, while the latter is found in...
Fatty acyl reductase (FAR) enzymes catalyze the reduction of fatty acyl-coenzyme A (CoA) or fatty acyl-acyl carrier protein substrates to primary fatty alcohols. FAR enzymes have distinct substrate specificities with regard to chain length and degree of saturation. FAR5 (At3g44550) and FAR8 (...