After removing the MTT solution, dimethyl sulphoxide was added to each well and aspirated to dissolve all of the crystals and then left at room temperature for a few minutes to ensure Scientific Reports | 5:15977 | DOI: 10.1038/srep15977 9 www.nature.com/scientificreports/ the solubility of...
MTT dye has been selectively reduced by the viable cells into insoluble dark purple colored formazan crystals. In brief, 104 L-132 cells stably expressing GFP/CD::UPRT gene were seeded in 96 well plate (Corning, Costar, NY), while for control (non-transfected) L-132 cells were taken. ...
where C—constant characteristic for quartz crystals; ΔF—change in the resonance frequency; and n—number of overtones (in the present case, n = 7). 3.8. Contact Angle Measurements The hydrophilicity/hydrophobicity of the layers formed by the BSA-5FU complexes on the surface of the QCM-D...
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The albumin heterogeneity does not make it possible to obtain high-quality protein crystals suitable for a high-resolution X-ray diffraction analysis. Nevertheless, great efforts to obtain more than half a dozen different crystalline forms of SA contributed to the identification of the X-ray structur...
The crystals are next dissolved in an organic solvent (such as dimethyl sulfoxide (DMSO)) and such formed solution may be subsequently investigated via the UV-Vis spectroscopy. The concentrations of obtained solution corresponds then to the amount of the enzyme present in the tested medium and so...
After decanting, the resulting residue was washed with distilled water, crushed with diethyl ether, and dried under a vacuum to obtain yellowish-green crystals. Yield: 70%. M.p.: 114–117 ˚C. Vis λmax (DMF): 703 nm; Vis λmax (DMSO): 707 nm; Vis λmax (PB): 705 nm; Vis ...
Crystals of IrPNr·2CHCl3 suitable for X-ray analysis were obtained under refrigeration by slow evaporation of chloroform/acetone (1:1, v/v) solution under normal oxygen conditions. Crystal data: C41H46Cl8FIrN3O3P, M = 1154.58 g mol−1...
Formed formazan crystals are then dissolved in DMSO (or isopropanol) while the color intensity of this solution is evaluated spectrophotometrically and corresponds to the amount of formed formazan crystals and thus to the amount of the enzyme secreted by the tested cells (and thus corresponds to ...
Yes, several times larger individual cubic crystals were observed among the replicas, not associated with lacy structures (Figure 5a). Figure 5. (a,b) SEM images of large aggregates formed on the HOPG with different magnifications, obtained in the SE mode. Rectangles and arrows indicate ...