Firefly luciferase intensity was normalized to renilla luciferase intensity, and TCF/LEF reporter activity Lithium requires AKT in mood-related behavior models JQ Pan et al was reported as the fold-change over the control treatment (NaCl, control transfection). Wnt-conditioned media is prepared from...
. For bioluminescent imaging, mice anesthetized with isoflurane were imaged at 15, 20 and 25 min after intraperitoneal administration of firefly luciferin (150 mg/kg; Caliper Life Sciences) to capture maximal luminescence. Data acquisition and analysis were achieved using the Living Image® software...
The constitutive firefly luciferase (hluc þ ) expression was used as an internal normalization control (c). The Akt3 30 UTR or Akt3 30 UTR Mut (containing a 2-base substitution in the binding site for miR-29) construct was co-transfected with miR-29a, miR-29b, miR-29c, or NC, ...
Renilla luciferase activity was measured and normalized to firefly luciferase activity. F The total RNA m6A level after DAA treatment was detected by m6A RNA dot blot assay and compared with DMSO treatment, with methylene blue staining as a loading control. G qPCR was used for the detection of...
cZNF215 was overexpressed in HuCCT1 cells by lentiviral transfection and knocked down through short hairpin RNA (shRNA) in RBE cells, which were labeled with firefly luciferase allowing for tracking by the in vivo imaging system (IVIS). As a result, tumor growth was markedly promoted by cZN...
Once attached, MCF7 and ZR-75-1 breast cancer cells were transfected with pcDNA3.1-rLuc-PolioIRES-fLuc, which directs cap-dependent translation of the Renilla luciferase gene and cap-independent Polio IRES-mediated translation of the firefly luciferase gene. After 24 h, the transferred cells ...
Twelve hours later, transfected cells were transferred into GM, harvested, and firefly luciferase activity was determined using a Luminometer with Luciferase Reporter Assay System (Promega, Sunnyvale, CA, USA). Experiments were performed in triplicates and repeated at least three times independently. ...
Firefly lucifer- ase activities were standardized to those of b-galactosi- dase activity. Chromatin immunoprecipitation assay To detect the in vivo association of nuclear proteins with rat MMP-9 promoter, chromatin immunoprecipi- tation (ChIP) analysis was conducted as previously described [27]. ...
Then the firefly luminescence was measured in a luminometer. After that, a volume of 100 μL Dual-Glo Stop & Glo Reagent was added. Waiting for at least another 10 min, Renilla luminescence was measured and the luminescence ratio of HYD-PEP06 group to the control group was calculated. ...
The PI3K/AKT pathway plays an essential role in tumour development. NOD-like receptors (NLRs) regulate innate immunity and are implicated in cancer, but whether they are involved in PI3K/AKT pathway regulation is poorly understood. Here, we report that NLRP6 potentiates the PI3K/AKT pathway by...