内源性验证 Western blot analysis of extracts from serum-starved NIH-3T3, untreated (-); treated withPDGFA (Cat: 13081-H07Y) (5 μg/mL, 5 min; +), using Phospho-AKT (Ser473) rabbit monoclonal Antibody (Cat: 110591-R0113) at 1:2000 dilution (upper) or anti-Akt antibody (lower). ...
[15] Jindra PT, Jin YP, Rozengurt E, et al. HLA class I antibody-mediated endothelial cell proliferation via the mTOR pathway. J Immunol. 2008, 180(4):2357-2366. [16] Kendall RT, Lee MH, Pleasant DL, et al. Arrestin...
The transferred polyvinylidene fluoride membranes were incubated overnight with anti-FLAG M2 monoclonal antibody (Sigma, St Louis, MO, USA), anti-actin antibody (AC-40; Sigma-Aldrich), anti-Akt antibody (Cell Signaling Technology, Danvers, MA, USA), and anti–phospho-AKT ser473 (pAKT) ...
anti-Ki-67 antibody (Cell Signaling Technology, 9027, 1:800) diluted in normal horse serum for overnight at 4 °C. Sections were then washed with PBST buffer for 5 min and incubated with Goat anti-rabbit IgG (1:500) for 15 min at room temperature. After washing with PBST, sect...
中文名称:Anti-Akt(pan) Monoclonal Antibody英文名称:Anti-Akt(pan) Monoclonal Antibody Gene ID:207;208;10000Public Immunogen Range:Purified recombinant human AKT1 protein fragments expressed in E.coli. Subcellular Locations:Cell membrane Cytoplasm NucleusSwiss Prot:P31749;P31751;Q9Y243 ...
Antibody 克隆号 ZA006 抗原 Synthetic peptide derived from the C-terminal region of the human Akt2 protein, which is 95% and 90% homologous with rat and mouse, respectively 查看免疫原 偶联物 Unconjugated 形式 Liquid 浓度 0.5 mg/mL 纯化类型 Protein A 保存液 PBS, pH 7.4...
-p44/42 MAPK (Thr202/Tyr204) mAb is used to immunoprecipitate active p44/42 MAP kinase from cell extracts, then an in vitro kinase assay is performed using Elk-1 protein as a substrate. Elk-1 phosphorylation is then detected by Western blotting using Phospho-Elk-1 (Ser383) Antibody....
Our internal testing has confirmed that it reacts with the human and mouse forms of phospho-AKT.Reactivity with the human form is also supported by theliterature's citations of this antibody. References: 1.Perturbations of the AKT signaling pathway in human cancer. ...
signaling pathways in cells with TMEM176B overexpression and silencing. Silencing TMEM176B or inhibiting it with a therapeutic antibody impaired cell proliferation, while overexpression increased proliferation in vitro. Syngeneic and xenograft tumor studies revealed the attenuated growth of tumors with TMEM...
本Phospho-Akt(Ser473)抗体(Phospho-Akt(Ser473)antibody)为进口分装,用人工合成的小鼠Akt Ser473附近的一段多肽进行适当修饰后免疫rabbit,然后用protein A和抗原多肽亲和柱经过两步纯化得到的高纯度抗体。 本Phospho-Akt(Ser473)抗体识别Ser473被磷酸化的Akt1。相应位点被磷酸化的Akt2和Akt3也可以被本抗体识别。未...