每孔上样约30 μg蛋白, 经 10%SDS-PAGE 电泳后,采用湿转法 300 mA 转膜 2.5 h.以 5% BSA 封闭液室温封闭 1 h 后,分别加入小 鼠抗 GAPDH,Akt 抗体,兔抗 GFAP,p-Akt 和 p-ErbB2 抗体,4 ℃孵育过夜.二抗室温孵育2 h后,使用超敏发 光法检测蛋白表达,凝胶图像分析仪采集化学发光信 号.使用 ImageJ...
HME and BME samples were pre-diluted 1:100 in phosphate-buffered saline (PBS, 0.01 M) and loaded into a 1 mL syringe. The measurements were carried out using a nanoparticle tracking analyzer (NTA; NanoSight NS300, Malvern, UK), during which the samples were analyzed under scattered light ...
The final R1 values = 0.0332 (I > 2σ(I)), wR(F2) values = 0.1116 (I > 2σ(I)), R1 values = 0.0334 (all data), and wR(F2) values = 0.1118 (all data). The goodness of fit on F2 = 1.044. Flack parameter = 0.023(4). m.p. 181 ...
(2)热电偶:WR25型、B型、E型、J型、K型、S型、T型、R型; (3)热电阻:Pt100、Cu50; (4)根据用户要求定制。 输出规格 *多8路继电器输出,250VAC 3A,30VDC 3A,默认为常开触点 累积范围 0~4294967295; 通讯、打印 通讯接口:RS232C或RS485;波特率:1200、2400、9600、19200 打印接口:RS232C直接连接串行...
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Valvassori SS, Dal-Pont GC, Resende WR, Jornada LK, Peterle BR, Machado AG et al (2017) Lithium and valproate act on the GSK-3β signaling pathway to reverse manic-like behavior in an animal model of mania induced by ouabain. Neuropharmacology 117:447–59 Article CAS PubMed Google Scho...
Friedenberg WR, Rue M, Blood EA, et al. Phase III study of PSC-833 (valspodar) in combination with vincristine, doxorubicin, and dexamethasone (valspodar/VAD) versus VAD alone in patients with recurring or refractory multiple myeloma (E1A95): a trial of the Eastern Cooperative Oncology Group....
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