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AKR1C1 antibody (GTX105620, GeneTex, Irvine, California, USA) was diluted in 1:2000. Images were photographed by an inverted microscope (Leica DMI 4000 B, Wetzlar, Germany). Further analyses were conducted by Image-Pro Plus software. (Normal lung tissue: n = 8; Cancerous lung tissue: ...
The standard of care for advanced EOC consists of radical cytoreductive surgery followed by adjuvant platinum-based chemotherapy and maintenance targeted therapy such as anti-angiogenic antibody, bevacizumab, or poly-ADP-ribose-polymerase inhibitors4. Even though initial response rates are between 60 and...
After dewaxing, rehydration and thermal demasking of the tissue slides, AKR1C1/2 expression was visualized using a polyclonal rabbit IgG (dilution: 1:100, 16 h at 4 °C) (abcam, Cambridge, UK) as primary antibody and the ZytoChem-Plus HRP Polymer-Kit (Zytomed Systems GmbH, Berlin...