We found that AD patients showed a decrease in the abundance of Akkermansia muciniphila and a decrease in propionic acid both in fecal and in blood. In order to further reveal the effects and the mechanisms of propionic acid on AD prevention, we systematically explored the effects of propionic...
The interplay between gut microbiota and immune responses is crucial in ulcerative colitis (UC). Though Akkermansia muciniphila (Akk) shows therapeutic potential, the mechanisms remain unclear. This study sought to investigate differences in therapeutic
muciniphila (cell to bacteria ratio, 1:20) or cell-free supernatant (CFS) (10% v/v) (ATCC BAA-835 or SNUG-61027). b, GLP-1 secretion by NCI-H716 cells after treatment with various amounts of A. muciniphila (10–100% v/v). c, GLP-1 secretion after the treatment of NCI-H...
The interplay between gut microbiota and immune responses is crucial in ulcerative colitis (UC). Though Akkermansia muciniphila (Akk) shows therapeutic potential, the mechanisms remain unclear. This study sought to investigate differences in therapeutic
A. muciniphila(ATCC BAA-835) was cultured under anaerobic conditions (99% N2at 37 °C) until the optical density at 600 nm reached 1.5 as previously described.7The isolation of EVs was performed as previously described.23Briefly, bacterial cultures were pelleted at 10 000 gfor 20...
5.2. Culture of Akkermansia muciniphila Akk (ATCC-BAA-835) was cultured with modifications to previously described methods (Yoon et al., 2021, Derrien et al., 2004). Briefly, Akk was cultured in anaerobic incubator (LAI-3T, LONGGYUE, China) for 48 hours in a media containing various ...
A. muciniphila(cell to bacteria ratio, 1:20) or cell-free supernatant (CFS) (10% v/v) (ATCC BAA-835 or SNUG-61027).b, GLP-1 secretion by NCI-H716 cells after treatment with various amounts ofA. muciniphila(10–100% v/v).c, GLP-1 secretion after the treatment of NCI-H716 ...
Akkermansia muciniphila (A. muciniphila), an intestinal symbiont colonizing in the mucosal layer, is considered to be a promising candidate as a probiotics. Most of the current researches focus on th...
A. muciniphila(ATCC BAA835) was cultured under anaerobic conditions at 37 °C in Brain Heart Infusion media supplemented with 0.05% hog gastric mucin-type III (Sigma-Aldrich, St. Louis, MO, USA) [29].A. muciniphilawas freshly cultured in anaerobic conditions as described above for about...
A. muciniphila ATCC BAA-835 was streaked out from À 80 °C on BD Brain Heart Infusion (BHI) agar supplemented with 0.4% mucin (Sigma) and incubated under anaerobic conditions using the GasPack 100 system (BD Biosciences) at 37 °C for 36 h. Bacterial colonies were swabbed from the...