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Airway epithelia express MCT2 and MCT4. Immunostaining revealed no expression of MCT1 in pri- mary HBE cells and little, predominantly intracellular staining for MCT1 in H441 cells (Fig. 4A–D). MCT2 was clearly immunostained in western blot as a ~40 kDa protein and was present ...
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In our well-differentiated cells, α2,6-linked sialic acid is predominantly expressed on the apical surface and the basal cells mainly express α2,3-linked sialic acid. During the whole infection period, release of infectious virus was maintained at a high titre for more than seven days. The...
Airwaysmooth musclecells are a subset of mesenchymal cells that are distinct fromvascular smooth muscle cellsalthough both lineages express myogenic markers such as Myh11 and Cnn1. Differentiation ofairway smooth muscle cellsfrom embryonic lung mesenchymal progenitors occurs earlier than vascular smooth mus...
( figure s5 f). this suggested that rv-infected ciliated cells efficiently transmit a signal to the non-ciliated cells, which then express genes in asthma-risk loci. the time-course experiments indicated this upregulation of asthma-associated genes occurred predominantly at 24 and 42 h. 42 in...
Interestingly, only 71 mRNAs were identified to express differentially between MSCs-CD4+ T cells and IL-10-MSCs-CD4+ T cells (Fig. 7A). Among the 71 differentially expressed mRNAs, 12 were upregulated and 59 were decreased in IL-10-MSCs-CD4+ T cells compared to those in MSCs-CD4+ T ...
EGFP(Foxi1)+ cells express canonical airway markers Ttf1 (Nkx2-1) and Sox2 (solid outlines). EGFP(Foxi1)+ cells do not label with basal (Trp63), club (Scgb1a1), ciliated (Foxj1), tuft (Gnat3), neuroendocrine (Chga) or goblet (Tff2) cell markers (dashed outlines). b, Ionocy...
(Supplementary Fig.2d, Fig.1e), we generated mice with selectiveCD47loss in ciliated cells by creatingCD47floxed mice (CD47f/f), bred to constitutively express Cre under the control of the Foxj1 promoter (Foxj1Cre)48. Cre specificity was validated by crossing with tdTomato reporter mice (...