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Lyo-Ready™ & Air-Dryable™可冻干 & 可风干 LAMP/RT-LAMP 预混液 在过去的十年中,等温扩增技术,例如杂交链式反应(HCA)、多位移扩增(MDA)和环介导等温扩增(LAMP)等在即时诊断(POC)中发挥了越来越重要的作用。Lyo-Ready ™ & Air-Dryable ™ 可冻干 & 可风干 LAMP/RT-LAMP 预混液在过去的十年...
metabolism. In contrast, the heat-associated increase in VPD (dry air) accounts for the majority of metabolomic heat stress responses. Since dry air might provoke drought in leaves, we expect that the metabolomic response partly resembles that of a drought stress scenario. Indeed, 21% of the m...
RT-qPCR reaction was carried on the Applied Biosystem 7500 real-time PCR system, using 2 × SYBR Green qPCR Mix produced by Aidlab Biotechnologies Co., Ltd. (Beijing, China). RT-qPCR was performed under the following conditions: 95 °C for 3 min, 40 cycles at 95 °C for 15 s, 60 ...
mm fractions, but not on the 'ultra' detection for this assay, which is in the order of 1–10 fraction (o5 mm) in the Mississippi River plume, total copies; we also note that this may partially be suggesting that microzooplankton are better able to due to the late-day sampling time....
Throat, nasal and rectal swabs were collected using dry swabs (Coban, cat. 155CS01) every other day, to prevent cross-contamination, until they were negative for SARS-CoV-2 RNA or maximum for 21 dpi/dpe by determined by real-time RT-qPCR as described below. Swabs were stored at −80...
Table 1 Primers used for qPCR assays withnirK,nirS,nosZI,nosZII,nrfAand 16 S rRNA genes as targets Full size table Each qPCR reaction consisted of Luna®Universal qPCR Master Mix (New England BioLabs GmbH, Frankfurt am Main, Germany), forward and reverse primer, bovine serum albumin (BSA...
将风干后的 Air-Dryable™ 可风干LAMP预混液( 红色 )和其液体试剂( 蓝色 )以及NEB WarmStart® 的液体试剂( 橙色 )进行性能对比,结果显示对所有 扩增的模板中可风干LAMP预混液的灵敏度更高,反应时间更快,TTR更短。A)使用Air-Dryable™ 可风干LAMP预混液(MDX119)的风干试剂( 红色 ) 或液体( 蓝色 ...