酶联免疫斑点技术(EnzymeLinked ImmunospotAssay,ELlSPOT)是细胞免疫学研究中敏感的检测方法之一,可以在单细做水平对抗体分泌细胞(ASC)及细胞因子(CK)分泌细胞进行检测。由于是单细胞水平检测,ELISPOT 比ELISA和有限稀释法等具有更高的灵敏性。能从20万-30万细胞中检出1个分泌该蛋白的细胞。并且该方法能够对抗原刺激后...
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e, western blot of MNs (26-28DIV) from two healthy controls, transduced with LV-shRen (n = 5) or LV-shEx4 (n = 6), probed with anti-total-tau and anti-GAPDH antibodies. Quantification is shown on the right (mean ± s.d. *P < 0.05, two-sided Wilcoxon-test). Source data...
All three sera failed to react in one antiglobulin enzyme-linked immunosorbent assay (ELISA) (Bioelisa HIV-1+2, Biokit SA). No single underlying reason could be identified for the assay failure in the three cases. The first patient, probably infected recently when first tested, was strongly...
Induction by anti-CD40 antibody or soluble CD40 ligand and cytokines of IgG, IgA and IgE production by B cells from patients with X-linked hyper IgM syndrome Eur. J. Immunol., 23 (1993), pp. 2294-2299 CrossrefView in ScopusGoogle Scholar 20 A Durandy, C Hivroz, F Mazerolles, C Sch...
0801006 Anti-HIV Type 1 gp41 Clone 10E9 (100 µg) Immunology Critical ID Antibodies Monoclonal RUO 0801007 Anti-HIV Type 1 RT Clone 39/4.12.2 (100 µg) Immunology Critical ID Antibodies Monoclonal RUO 0801008 HIV Type 1 p24 Antigen ELISA 2.0 (5 X 96 Determinations) Immunology Critical...
The procedure is based on the comparison of known concentrations of IgG or IgA bound to anti-F(ab)'(2) with the binding of an unknown concentration of antibodies to a specific antigen. We applied the new assay to the measurement of HIV1-gp160 specific IgG and IgA concentrations in ...
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+: resistant to HIV1 challenge +/-: partially resistant -: not resistant These results are encouraging in that in some cases, the nucleic acid construct, which can encode potential anti-sense RNA for interference hybridization, showed significant anti-viral protection. However, one third of the ...