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Approximately, 6 μg each of Gαi antibody (sc56536, Santa Cruz Biotech), SpVasa antibody24, or suNuMA antibody #1 (peptide antibody constructed by Genescript, HK) was used per sample for immunoprecipitation (IP). The IP was performed as described in the instruction manual of Dynabeads ...
C: 1 – 5 mA DC (Load resistance 3000 Ω max.) D: 0 – 20 mA DC (Load resistance 750 Ω max.) E: 0 – 16 mA DC (Load resistance 900 Ω max.) F: 0 – 10 mA DC (Load resistance 1500 Ω max.) G: 0 – 1 mA DC (Load resistance 15 kΩ max.) ...
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mass spectrometer was performed in positive ion mode with the following source parameters: drying gas temperature—200 °C, with a flow of 14 L/min, nebulizer gas pressure—30 psi, sheath gas temperature—400 °C with a flow of 11 L/min, capillary voltage—3000 V and nozzle voltage—800 ...