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AGRP多克隆抗体(Agrp polyclonal antibody)是一种针对AGRP(Agouti-related protein,又称Agrt或Art)蛋白的特异性抗体。它属于多克隆抗体的一种,即由多个不同的免疫细胞克隆所产生的抗体 AGRP多克隆抗体(Agrp polyclonal antibody)是一种针对AGRP(Agouti-related protein,又称Agrt或Art)蛋白的特异性抗体。它属于多克隆抗...
Slices were incubated in goat anti-AgRP antibody (1:1000, Santa Cruz Biotechnology) for 3 h at room temperature, then washed and incubated with donkey anti-goat Alexa568 secondary antibody (1:400, Invitrogen) for 2 h at room temperature, then mounted on glass slides for imaging. ...
1% bovine serum albumin, 0.3% triton-X 100 in PBS) for 1 h at room temperature followed by incubation with anti-c-Fos antibody (1:250, #sc-166940 X, Santa
We processed mouse brains and cut 10-μm-thick coronal sections for immunohistochemistry as described (Plum et al., 2009) by using pAkt (Cell Signaling 4060), pStat3 (Cell Signaling 9131), and pS6 antibodies (Cell Signaling 4858) and also Foxo1 antibody (Santa Cruz, FKHR-H128, sc11350)....
Finally, sections were incubated with Alex Fluor 488-conjugated donkey anti-rabbit secondary antibody (1:1000 dilution; 711-545-152, Jackson Immunolab, West Grove, PA), or Alex Fluor 488-conjugated goat anti-chicken secondary antibody (1:1000 dilution; A-11039, ThermoFisher Scientific, Waltham,...
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Contrasting to the established role of the hypothalamic agouti-related protein (AgRP) neurons in feeding regulation, the neural circuit and signaling mechanisms by which they control energy expenditure remains unclear. Here, we report that energy expendi
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