depolarization of the membrane was recorded, a depletion of the intracellular ATP pool seemed to be the mainbactericidal effect. Therefore, it was concluded that mutacin II might act by inhibiting the generation of ATP in the cells, which, if proven, would be a novel mode of action for ...
Carbon footprint (CF) is a popular element in the agro-environmental sciences due to its role in the environmental impact assessments related to alternative solutions and global climate change. The International Organization for Standardization (ISO) 14001 certification is a starting point of prospectus...
In the late renaissance, around 1700, bothchemistryand physiology were emerging as defined sciences. A consequence of their overlap was the definition of a new science,biochemistry—the chemistry of living organisms, now largely freed from the constraints of vitalism. We shall see that biochemistry,...
ATPCd胁迫根超氧阴离子To evaluate the protective effect of exogenous ATP on the roots of mung bean exposed to Cadmium (Cd) ,the cell death degree of mung bean and 02 - of roots of mung bean were determined. The results showed that ATP (400 μM) added to the hydroponics solution conc...
Na+-K+-ATP酶通过测定鹿特异性复合麻醉剂麻醉下大鼠大脑皮层Na十-K+-ATP酶活性的变化,探讨了其麻醉与大鼠大脑皮层Na+-K+-ATP酶的关系.将32只纯种SD大鼠随机分成对照组,诱导组,麻醉组和催醒组,对照组大鼠按30 mL/kg体重腹腔注射生理盐水,其余各组按30 mg/kg体重腹腔注射鹿特异性复合麻醉荆.于各时间点冰上...
研究其对牛卵母细胞体外成熟及激活后孤雌胚胎发育能力的影响.[结果]在成熟培养液中添加50μg/ml尿嘧啶能够显著提高卵母细胞的成熟率,分裂率及孤雌胚胎囊胚率;在成熟培养液中分别添加0,250,500,750μg/mlATP,对卵母细胞成熟率影响不大,但添加5μg/ml ATP能显著提高激活后孤雌胚胎囊胚率.[结论]该研究为牛...
同源重组V型ATP酶VPH1蛋白制备V型三磷酸腺苷酶(ATP酶)是真核生物调节胞质内细胞器酸碱度的核心元件,对盐等胁迫环境下细胞膜的稳定起着重要作用.VPH1是V型ATP酶蛋白复合物膜上的1个亚基蛋白,其跨膜结构对V型ATP酶的功能有很大影响.本研究利用PCR(Polymerase chain reaction)介导的同源重组技术,构建毕赤酵母内源...
ATP合成酶基因克隆以多效唑诱导中国水仙的抑制差减杂交文库(SSH)获得的cDNA片段为基础,采用RACE及RT-PCR技术,分离克隆到1个ATP合成酶CF0 B亚基基因NtA TPF.此基因包含有1个555 bp完整阅读框架(ORF),编码184个氨基酸,具有1个跨膜区域(27～49),分子量为20.809 5 kD,理论等电点为5.69.聚类分析表明:其氨基酸...
ATP合酶β亚基克隆序列分析[目的]克隆刺五加叶绿体的ATP合酶β亚基cDNA并对其进行生物信息学分析.[方法]根据已知物种叶绿体ATP合酶β亚基基因的序列,设计1对同源引物,通过RT-PCR扩增得到刺五加ATP合酶β亚基cDNA,并对其进行序列比对及结构预测分析.[结果] RT-PCR扩增获得了长1 099 bp的刺五加ATP合酶β亚基cDNA,...
为更好地了解鲢鱼(Hypophthalmichthys molitrix)在微冻贮藏过程中的相关品质变化,对微冻条件(-3℃)下生鲜鲢鱼片品质指标,ATP关联产物及ATP关联酶活性的变化规律进行试验研究.结果表明:微冻条件下,挥发性盐基氮的质量分数呈现不断上升的趋势,但在30 d贮藏时间内未超过国家规定的二级新鲜度标准(20...