Column: Agilent ZORBAX Eclipse HILIC Plus narrow bore, 2.1 mm × 100 mm, 3.5 µm, p/n 959793-901 Column temp: 25 °C Mobile phase: 5% ACN and 5% H2O with 10 mM ammonium acetate, pH 6.5 Flow-rate: 0.25 mL/min Gradient: Injection volumes: t0 = 95% ACN t9 = 70% ACN t15 =...
Applying multiple heart-cutting enabled a fully automated HILIC-based lipid (sub-)class type fractionation in the first dimension and online transfer of the fractions onto a high-resolution reversed‑phase UHPLC column in the second dimension for separation of group members according to ...