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Microglia, the resident immune cells of the brain, play a crucial role in maintaining homeostasis in the central nervous system (CNS). However, they can also contribute to neurodegeneration through their pro-inflammatory properties and phagocytic functio
(Bio-Rad, Hercules, CA, USA). All protein bands were quantified using ImageJ software (National Institutes of Health, NIH, Maryland, MD, USA). The data obtained were normalized to the β-actin (Cell signaling, Danve...
The results revealed that hypo-DMRs are significantly enriched for Ring1b, Scl, Gata1, Ldb1, and Runx1 binding sites in old HSCs but not for Rad21 binding sites, which served as a negative control (Figure 6A). Combinatorial interactions of Scl, Lyl1, Gata2, Runx1, Lmo2, Fli1, and ...
The immunoblots were imaged with ChemiDoc TM MP system (Bio-Rad) and the optical density of the bands were quantified in the Image Lab program (Bio-Rad). Loading an equal volume of isolated EVs is a common practice for characterization of EVs markers with Western blot. According to the ...
Control of replication stress and mitosis in colorectal cancer stem cells through the interplay of PARP1, MRE11 and RAD51. Cell Death Differ. 2021;28[7]:2060-82. 31. Gorgoulis V, Adams PD, Alimonti A, Bennett DC, Bischof O, Bishop C et al. Cellular Senescence: defining a path ...
Institutional Animal Care and Use Committee BWH: Brigham and Women’s Hospital PBS: Phosphate-buffered saline T-per: Tissue protein extraction reagent ROI: Region of interest DG: Dentate gyrus IPA: Ingenuity pathway analysis Nfkb2: Nuclear factor kappa B subunit 2 ...
assessed using the Total OXPHOS antibody (ab110413) at a concentration of 1:2000 purchased from Abcam Inc. (Cambridge MA, USA). Quantification was analyzed using a densitometer (Bio-Rad Laboratories, Hercules, CA, USA) and normalized by total protein determined using 0.1% amido-black (Sigma-...
Oocyte aging is a key constraint on oocyte quality, leading to fertilization failure and abnormal embryonic development. In addition, it is likely to generate unfavorable assisted reproductive technology (ART) outcomes. SCM-198, a synthetic form of leonu
Fluorescence was measured using a Bioplex system (Luminex 100, BioRad, Hercules, CA). More details are provided in Song et al.17. Cardiovascular Phenotypes and Diabetes. Diabetes, myocardial infarction, stroke, hypertension, cho- lesterol, HDL and LDL cholesterol levels were assessed. ...