If the migration occurs through a gel matrix of carefully chosen pore size, the mobility of the RNA molecules is related to the logarithm of the molecular weights: the smallest molecules moving the greatest distance. Suitable gel matrices for the electrophoresis of RNA are polyacrylamide or agarose...
RNA molecular weight markers, an RNA sample known to be intact, or both, can be used for this purpose. Ambion's NorthernMax reagents for Northern Blotting include everything needed for denaturing agarose gel electrophoresis. These products are optimized for ease...
The overall quality of an RNA preparation may be assessed by electrophoresis on a denaturing agarose gel; this will also give some information about RNA yield.
RNA-PAGERapid and correct diagnosis of rotavirus infection is very important to offer immediate treatment. In the present study, ribonucleic acid-polyacrylamide gel electrophoresis (RNA-PAGE) and agarose gel electrophoresis (AGE) methods were standardised and compared for the detection of rotaviruses. ...
中文: 总RNA的提取:在0g/L琼脂糖凝胶电泳上,可见8s和8s两个清晰条带,8s与8s比值约为∶,未见明显降解。 更详细... 英文: Methods of Agarose Gel Electrophoresis for mRNA Differential Display 中文: 琼脂糖电泳展示mRNA差异方法的探讨 更详细... 英文: Factors of Agarose Gel Electrophoresis which Influ...
摘要: INTRODUCTIONPerhaps the most important and certainly the most often used technique in RNA analysis is gel electrophoresis. Because RNAs are negatively charged, they migrate toward the anode in t ...DOI: 10.1101/pdb.prot5445 被引量: 25 ...
her DNA samples. An image of a gel post electrophoresis. EtBr was added to the gel before electrophoresis to a final concentration of 0.5 μg/ml, followed by separation at 100 V for 1 hour. The gel was exposed to uv light and the picture taken with a gel documentation system.货号:
Gel and PCR clean-up kits Agarose gel electrophoresisWe offer agarose gel powders, DNA and RNA Markers, plus gel electrophoresis instruments and accessories for your DNA separation needs. Select from the following gel electrophoresis products:Product...
agarosegelelectrophoresissrm琼脂电泳 EXPERIMENTNO:9(B) DATE: AGAROSEGELELECTROPHORESIS AIM: ToanalyzethegivennucleicacidusingAgaroseGelElectrophoresis. MATERIALSREQUIRED: 1.Agarosesolution, 2.Ethidiumbromide, 3.Electrophoresisbuffer, 4.6xgelbuffer, 5.DNAsample, 6.DNAsizestandard. PRINCIPLE: Agarosegelelectroph...
Data 3: Electrophoresis in Mini-gel (Mupid® ) run at 50V for 80min. in TAE Buffer 3% Agarose 21 Lane 1: 0.1 µg DNA Marker / lane Lane 2: 0.5 µg DNA Marker / lane M4 : Marker 4 (φX174/Hae III digest) M5 : Marker 5 (φX174/Hinc II digest) ...