DNA fragment size Gel percentage Agarose per 50 mL 800-12,000 bp 0.8% 0.4 g 500-10,000 bp 1% 0.5 g 400-7000 bp 1.2% 0.6 g 200-3000 bp 1.5% 0.75 g 50-2000 bp 2% 1g Note: The final concentration of GelRed® in a 1% gel is 1X. The GelRed® concentration will vary with...
Average particle size 45-165μm Coupled Ligand Biotin M.W. of Ligand 244Da (Biotin) Ligand concentration 1~1.5μmol Biotin per ml settled gel Binding capacity Per ml settled gel: 10-20mg streptavidin Specificity (Strept)avidin coupled ligands Elution method Elution with acid or SDS-PAGE loading...
The electrophoretic mobility of single-stranded DNA (ssDNA) through agarose gels depends on size (number of bases; molecular weight), topology (linear or circular), and conformation (secondary and tertiary folding through base pairing and stacking), as well as on gel concentration and field ...
Slater G.W., Turmel C., Lalande M., and Noolandi J.(1989) DNA gel electrophoresis: effect of field intensity and agarose concentration on band inversion. Biopolymers 28 , 1793–1799. View ArticleG. W. Slater, C. Turmel, M. Lalande, and J. Noolandi, “DNA Gel Electrophoresis: Effect...
DNA Gel Electrophoresis is a technique used to separate and identify DNA fragments based on size. DNA fragments of various sizes are loaded into a porous gel made from agarose – a carbohydrate found in red algae. When an electric field is applied, the fragments will migrate through the gel,...
Agarose gel electrophoresis remains the most widely used technique for separating nucleic acid fragments due to its ease of use, non-toxicity, and broad separation range. By varying agarose concentration, gel pore size can be controlled to separate nucleic ...
Electrophoresis using E-Gel SizeSelect II Agarose Gels is a convenient method for accurate size selection of DNA libraries for next-generation sequencing (NGS) applications. With E-Gel SizeSelect II gels, you can separate and recover DNA for library construction in three easy steps: load, run,...
Average particle size45-165μm Coupled proteinStreptavidin M.W. of protein~55kDa (Streptavidin) Protein concentration≥2mg streptavidin per ml settled gel Binding capacityPer ml settled gel: ≥1mg biotinylated antibody or dsDNA, ≥200nmol free biotin, ≥8nmol biotinylated oligonucleotides or peptides...
Acrylamide cannot be used for this purpose, because it remains liquid at the concentration required for the appropriate separation of high-molecular-weight analytes. The movement of molecules through an agarose gel is dependent on the size and charge of separated particles, as well as the pore ...
Before casting an agarose gel, consult Table 2.1 to determine the appropriate percent agarose gel to use based on the size of DNA to be separated. Procedure 1. Determine the amount of agarose (grams) and volume needed. Use Tables 2.1 and 2.2 as a guide for agarose concentration and gel ...