Methods Mononuclear cells were collected by leukapheresis and enriched for monocytes by either counterflow centrifugal elutriation, or immunomagnetic selection using Isolex, an automated closed-system device. Monocytes were cultured for 7 days in serum-free medium with GM-CSF and IL-4, using either ...
Taner T, Hackstein H, Wang Z, Morelli AE, Thomson AW. Rapamycin-treated, alloantigen-pulsed host dendritic cells induce ag-specific T cell regulation and prolong graft survival. Am J Transplant. 2005; 5 :228.Taner T, Hackstein H, Wang Z, Morelli AE, Thomson AW (2005) Rapamycin-treated...
Introduction Protocol Results Discussion Disclosures Acknowledgements Materials References English Automatically Generated Tags Stem CellsIPSCViral Antigen-specific T CellsHBV ReplicationCD8-positive T CellsAdoptive Cell TransferCell CultureFlow CytometryCytokinesPlasmid DeliveryTail Vein InjectionCulture MediumSplenocyte...
2+ T cellsObjectiveTo investigate immune responses of peptide-specific CD4+ and CD8+ T cells, and nonpeptide-specific Vγ2Vδ2+ T cells during clinical quiescence of latent Mycobacterium tuberculosis coinfection in HIV-1-infected humans.
Immunosuppressive drugs on induc- ing Ag-specific CD4+ CD25+ Foxp3+ Treg cells during immune response in vivo. Trans- pl Immunol 2012; 27:30-8.Wu T, Zhang L, Xu K, Sun C, Lei T, Peng J, et al. Immunosuppressive drugs on inducing Ag-specific CD4(+)CD25(+)FoxP3(+) Treg cells ...
Volume 203, Number 1 (2000), in the article LFA-3 Plasmid DNA Enhances Ag-Specific Humoral- and Cellular-Mediated Protective Immunity against Herpes Simplex Virus-2 in Vivo: Involvement of CD4+ T Cells in Protection, by Jeong-Im Sin, Jong Kim, Kesen Dang, Dan Lee, Catherine Patchuk, C...
Cells were pelleted, frozen at −70°C, thawed in NTEN150 (20 mM Tris-HCl pH 8.0, 1 mM, 150 mM NaCl, 0.5% (v/v) Nonidet P-40), and sonicated. Extracts were centrifuged twice at 10,000 × g for 15 min. All of the glutathione S-transferase fusion proteins were soluble. The...
In order to determine which cells of the odontoblast-pulp complex were responsible for the making of AG1, in situ hybridization was carried out using digoxigenin-labeled probes. The full length AG1 cDNA was subcloned into the pBluescript vector, which contains two strong promoters, T3 and T...
The leaf specific expression of nitrate transport proteins leads to an increased phloem discharge of nitrate ions and to an increased nitrate uptake into leaf parenchyma cells. The N-reduction in plastids is increased by elevated nitrate accumulation in leaf parenchyma cells. The elevated transport of...
A complementary approach of antibody functionalization is the combination of different target specificities in one molecule to generate bi- or multispecific antibodies or antibody fragments, thus allowing alternative mechanisms of action, like the retargeting of T cells, as exemplified by bispecific antibody...