The disclosure also provides treatment methods based on inhibiting Fc纬RIIA or Fc纬RIIIA receptor signaling. Also provided are novel cell lines that are useful in measuring afucosylated Fc glycans.Taia T. Wang
A commonly used and efficient approach has been knocking out the FUT8 gene of the Chinese hamster ovary (CHO) host cells, which results in expression of antibody molecules with fully afucosylated glycans. However, a major drawback of the FUT8-KO host is the requirement for undertaking two ...
The disclosure also provides treatment methods based on inhibiting FcyRIIA or FcyRIIIA receptor signaling. Also provided are novel cell lines that are useful in measuring afucosylated Fc glycans.WANG, TAIA T.
The disclosure also provides treatment methods based on inhibiting Fc纬RIIA or Fc纬RIIIA receptor signaling. Also provided are novel cell lines that are useful in measuring afucosylated Fc glycans.TAIA T. WANG
A commonly used and efficient approach has been knocking out the FUT8 gene of the Chinese hamster ovary (CHO) host cells, which results in expression of antibody molecules with fully afucosylated glycans. However, a major drawback of the FUT8-KO host is the requirement for undertaking two ...
During both types of immunizations, antigen-specific immunoglobulins G (IgGs) have been characterized by the reduced amount of fucose present on N-linked glycans of the fragment crystallizable (Fc) region. These glycans are essential for mediating the induction of immune effector functions. ...
The recruitment of effector cells by mAbs is negatively affected by fucose modification of N-Glycans on the Fc; thus, utilization of afucosylated mAbs has been a trend for enhanced ADCC therapeutics. Most of afucosylated mAbs in clinical or commercial manufacturing were produced from Fut8-/-...
The structures reveal a unique type of interface consisting of carbohydrate–carbohydrate interactions between glycans of the receptor and the afucosylated Fc. ... C Ferrara,S Grau,Christiane Jaeger,... - 《Proc Natl Acad Sci U S A》 被引量: 502发表: 2011年 Pharmacokinetics of IgG1 monoclonal...
It is now widely recognized that removal of the core fucose from the N-glycans attached to Asn297 of human IgG1 significantly enhances its binding to its receptor, FcRIIIa, and thereby dramatically improves antibody-dependent cellular cytotoxicity (ADCC). Recent reports showed that removal of ...
Mass spectrometry analyses of the intact antibody and N-glycans show that β-carbafucose is not incorporated into the antibody N-glycans at detectable levels. We expect that β-carbafucose will serve as a useful research tool for the community and may find immediate application for the rapid ...