Enzymatic specimens were prepared using the Avita Medical ReCell system per manufacture instructions for use. Mechanical specimens were prepared using an abrasive pad until epidermis was macroscopically removed. Histologic analysis was performed with hematoxylin and eosin stain and whole slide scanning. Two...
Punch Biopsy Wound Care (With Stitches) Studies have proven that wounds heal faster when scabs are not allowed to form. Follow these instructions to prevent scabs from forming. This will result in better wound healing. Leave the original dressing in place for 24 hours Clean the wound once a ...
No studies have reported the effectiveness of using a mixture of gelatin sponge and hemocoagulase injection to block biopsy passages. The gelatin sponge can be prepared from pig skin, can be absorbed in the human body, and can be used for hemostasis and embolism. Hemocoagulase injection can be...
For protein assay, BALF was centrifuged at 200 × g for 5 minutes to pellet the cells; protein in the supernatant was measured with a bicinchoninic acid protein kit (Thermo/Pierce, Rockford, IL; 23235), according to the manufacturer's instructions using bovine serum albumin standards and read...
(MRI) study of the pelvis showed no evidence of osteomyelitis or a rectosacral fistula. The fever resolved. Because of the infected ulcer, the decision was made to defer induction chemotherapy for the acute leukemia, and he was discharged on the seventh hospital day, with instructions to take...
Briefly, the genomic DNA was extracted from a mycelial mass using a Fungal Microbial DNA Isolation Kit (Solarbio Life Sciences Laboratories, Beijing, PRC) in accordance with the manufacturer’s instructions. For this test, 2 μl of fungal DNA and 12.5 μl of the Taq Master Mix (Vazyme ...
The mouse was placed on a thermostatic station laying on the right side, shaved and disinfected with Povidone-iodine and an incision of 1–1.5 cm on the skin on the left side was performed, then the muscle layer below was cut and opened. The left kidney was then externalized pushing it...
Skin biopsy specimens revealed superficial and deep perivascular and either perieccrine or perineural lymphocytic infiltrates (Figure 1B). Immunohistochemical analysis of the infiltrates demonstrated a predominance of CD3+/CD56+/T-BET+/TCRγ3.20+/NKP46− cells consistent with γδ T cells (Figure 1...
Total RNA was extracted using TRIzol® (Life Technologies) as per the manufacturer’s instructions. Briefly, 2-3 mg of frozen liver tissue was pulverized under liquid nitrogen and 800 μl of TRIzol® was added to the resultant powder which was then homogenised with an Omni Tissue Homogenizer...
Then, samples were permeabilized using the PerFix EXPOSE (Beckman Coulter, Brea, CA) according to the manufacturer’s instructions and as previously reported [26]. Data were acquired using a fluorescence activated cell sorting (FACS) Canto II (Becton Dickinson) and were analysed with FlowJo v7.6...