Surrogate production by germline stem cell transplantation is a powerful method to produce donor-derived gametes via a host, a practice known as surrogacy. The gametes produced by surrogates are often analysed on the basis of their morphology and species
In males entry into meiosis is inhibited by signals from the developing testis, and male PGCs undergo mitotic arrest in G1 until puberty (Gondos and Hobel, 1971; =-=Bendsen et al., 2003-=-). Identifying the multiple stages of germ cell differentiation has mainly relied upon developmental ...
After editing, we isolated the individual blastomeres from 8-cell stage embryos and performed single-cell whole-genome sequencing (Fig. 1c). Because haplotype information markedly enhances copy number (CN) analysis in single-cell genomes, we used zygotes from a hybrid cross (129/SvJae x C57BL...
A discovery cohort of adult female childhood cancer survivors from the pan-European PanCareLIFE cohort (n = 743; median age: 25.8 years), excluding those who received bilateral ovarian irradiation, bilateral oophorectomy, central nervous system or total body irradiation, or stem cell transplantation...
Each horizontal bar represents one cell (n = 50). Grey: interphase; black: mitosis (from DNA condensation to anaphase); truncated bars: cell death. the cellular context remains an important and outstanding issue. Here we found that in human cells, MASTL determines the checkpoint and ...
Cells that contain 23 chromosomes, one of each pair, in humans are: A. diploid B. haploid C. gametes D. both B and C E. all of the above The inner cell mass of a blastocyst becomes the embryo. If two inner cell mass form, what will...
Each of these processes has been individually implicated in contributing to neuronal death; the possibility of their complex combinatorial interactions seems likely, but remains largely untested. In part because of the difficulties in symptom-based diagnosis and an incomplete knowledge of damage-inducing ...
Guanine-rich DNA, which has a potential to form a G-quadruplex (G4) structure, is particularly vulnerable to changes. In Caenorhabditis elegans, faithful transmission of G-rich DNA is ensured by the DOG-1/FANCJ deadbox helicase. Results: To identify a spectrum of mutations, after long-term...
Neither multivalent association nor pairing between homoeologous chromosomes was observed in meiosis in X. laevis (Tymowska, 1991), which implies that an ancient allotetraploid genome of X. laevis returned to a genetically diploid state through the process of diploidization of allopolyploid genomes. ...
Since Smurf1-regulated RhoA degradation is important for cell pro- trusive activity13, we investigated whether Smurf1-mediated RhoB degradation has the similar function. Same as previously reported13, overexpression of wild-type Smurf1 but not Smurf1- C699A resulted in a large number of long ...