The specimens were washed thrice with Hanks Balanced Salt Solution (HBSS), before being minced into small pieces, and digested with 3 mL sCelLiveTM Tissue Dissociation Solution (Singleron) by Singleron PythoN™ Tissue Dissociation System at 37 °C for 15 min. The cell suspension was collected ...
Neutrophils are traditionally viewed as first responders but have a short onset of action in response to traumatic brain injury (TBI). However, the heterogeneity, multifunctionality, and time-dependent modulation of brain damage and outcome mediated by n
iPSCs were digested enzymatically with 0.25% trypsin for 5 min in 37 °C. Single cells washed twice with cold DPBS and stained with conjugated SSEA1-PE with concentration of 1 μg/ml (Santa cruz: sc-21702 PE) for 15 min. Double positive for GFP and SSEA1 iPSCs were sorted into ...
The stomach pH also influences the transport of digesta. The acidity of the chyme leaving the stomach and arriving in the small intestine is decisive for the amount of digesta being transferred from the stomach to the small intestine. Acid-sensitive receptors provide feedback regulation to prevent ...
Sham mice were subjected to the same anesthesia and surgical procedures without being impacted. The dissected skull was put back and skin incisions in mice were sutured sterilely, and the mice recovered in clean and warm animal cages. The postoperative discomfort was reduced by increasing the ...
Although several attempts have been made to convert human and non-human primate PSCs into a truly naïve state, it is unclear which evaluation methods can discriminate them as being truly naïve. Here we attempted to derive naïve cynomolgus monkey (Cm) (Macaca fascicularis) embryonic stem ...
(Elementar Vario EL III; Elementar, Germany). To determine the TP content, samples were digested with 4:1 H2SO4/HClO442, and then the supernatant was passed through a 0.45-μm glass fiber filter (Q/IEF J01-1997, Shanghai) and analyzed using a continuous-flow analytical system (Skalar ...
However, all the feces had smaller polypeptides (< 15 kDa) that were not present in the CPI, which also indicates that the main cowpea proteins were digested during in vitro production of ICP and within the GIT. Most importantly, there were bands with > 200 kDa sizes in lanes 2...
Cells were then incubated in conditioned media for different periods of time (0, 3, 8, and 24 h) before being incubated with 2 mM bromouridine (BrU) at 37 °C for a 30 min. The cells were then lysed in TRIzol reagent (Invitrogen) and BrU-containing RNA was isolated as ...
In plants, an exceptionally high number of proteins are involved in Ubiquitin-26S proteasome system-mediated protein degradation and it is known to regulate most, if not all, important cellular processes. Here, we investigated the response to the inhibition of the proteasome at the protein level ...