The acentric chromosome fragment of the targeted chromosome 17 forms a micronucleus at the 4-cell stage and undergoes poor DNA replication. Fusion of the break ends of the centric fragments generates a dicentric chromosome. CN data suggest that this embryo, unlike the others, was harvested with ...
effect on pNRF2 levels (Supplementary Fig.S3c,g). Moreover, to further assess the effect of KD on regulation of oxidative stress, we evaluated p21 intensity levels. p21 is a cyclin dependent kinase inhibitor (CDK) that regulates multiple cellular processes such gene transcription, cell differentia...
When does DNA replication take place? A) During mitosis. B) Before a cell starts to divide. C) During cytokinesis. D) Continuously during the whole cell cycle. Secondary gamete precursor cells are: a. alloploid b. biploid c. celuloploid ...
proliferating cell nuclear antigen, DNA replication, recombination, mammals, DNA damage, dna, crossing overCompletion of DNA replication after replication stress depends on PCNA, which undergoes monoubiquitination to stimulate direct bypass of DNA lesions by specialized DNA polymerases or is poly...
Histone H3 methylation of lysine 79 occurs sequentially [4] and participates in key events involved in replication [5], cell cycle control [1], homologous recombination during double-stranded DNA repair, recombination during meiosis, and transcription initiation and elongation [6]; it therefore ...
In this model the nephron is damaged functionally by an ischemic reperfusion injury that results in regional areas of proximal tubule cell death. During the repair process the kidney proximal tubule epithelium undergoes a complex series of events involving 1) cell death and cast formation in the ...
This is probably due to the fact that HIV-driven CD4+ T-cell loss and dysfunction may lead to HCMV replication and subsequent expansion of CD8+ T cells. Indeed, an elevated number of CD8+ T cells and a low CD4+/CD8+ T-cell ratio have been observed in individuals co-in...
assay involves staining the cells with the green fluorescent dye CFSE, which enters live cells and stably binds to the proteins inside, resulting in permanent labeling. As a result, when the dye-containing parent cell divides, each daughter cell gets half the fluorescence from the parent cell. ...
As a model application we study the response of leukemia cells to the chemostatic drug vincristine as a function of cell cycle phase. The time-to-death after drug addition is found to depend both on drug concentration and cell cycle phase. The resulting timing and dose-response distributions ...
Together, these results show that upon AKI only a small TEC subset undergoes mitosis to replace no more than ∼50% of injured cells. Kidney tubules contain a distinct Pax2+ cell subset Using conditional Pax2.rtTA;TetO.Cre;R26.Confetti (Pax2/Confetti) mice (Supplementary Fig. 3a), we ...