coli cultures using Wizard® Plus Midiprep DNA Purification System (Promega, Mannheim, Germany). Biologics 2021, 1 54 4.3. RNA Synthesis and Binding of T7 RNA Polymerase The plasmid pcDNA3.1-VEGF was linearised with BstEII and used as a template for transcription by T7 RNA polymerase using...