Affinity chromatography is one of the most powerful techniques in selective purification and isolation of a great number of compounds. Affinity ligand is the most important factor in affinity chromatography, therefore, the selection and the screening of affinity ligand become the first issue to be ...
The various components of an affinity chromatographic method, including the types of affinity ligands, support materials, immobilization methods, and application or elution conditions that are employed in this technique, are discussed in this entry. Several specific types of affinity chromatography, ...
摘要: Affinity chromatography is a method of selectively and reversibly binding proteins to a solid support matrix based on the exploitation of known biological affinities between molecules. The interaction关键词: Affinity chromatography affinity ligand immobilization bioaffinity chromatography immunoaffinity ...
natural action,natural process,action,activity- a process existing in or produced by nature (rather than by the intent of human beings); "the action of natural forces"; "volcanic activity" paper chromatography- chromatography that uses selective adsorption on a strip of paper ...
A novel mode of affinity chromatography based on the open tubular column (OTAC) is presented in this paper. The OTAC column was prepared by coating a layer of Cibacron Blue F3GA onto the inner surface of capillary column with 50 microns inner diameter. The affinity chromatography experiment was...
Affinity Chromatography Since its inception in 1968, affinity chromatography has helped solve an increasing number of problems in the biomedical sciences. By this technique, a lar... I Parikh,P Cuatrecasas - 《Vox Sanguinis》 被引量: 206发表: 1972年 Handbook of affinity chromatography No Abstract...
Protein purification by affinity chromatography. Derivatizations of agarose and polyacrylamide beads The preparation of a number of agarose and polyacrylamide bead derivatives useful in the purification of proteins by affinity chromatography is described... Pedro Cuatrecasas - 《Journal of Biological Chemistry...
Isolation of multiple types by affinity chromatography on heparin-Sepharose. The mineralized matrix of osseous tissue harbors abundant mitogenic activity which is extractable by demineralizing solvents. In bovine bone powder free of... P.V. Hauschka,A.E. Mavrakos,MD Iafrati,... - 《Journal of ...
Despite over two decades of development the selective protein purification technique of affinity chromatography has only emerged recently as a viable contender for the purification of industrially significant proteins. This review describes some of the fundamental principles and applications of this powerful ...
Purification of enzymes by heparin-sepharose affinity chromatography Heparin selectively interacts with many enzymes and brings about significant changes in their activities. Due to its polyanionic nature, heparin also binds to many cationic proteins. Both these interactions may be used as a basis for ...