中文名称:HRP标记黄曲霉毒素B1(AFB1-HRP)/Aflatoxin B1-Peroxidase Conjugate 本制品是黄曲霉毒素B1(AFB1)与辣根过氧化物酶(HRP)经化学交联获得的偶联物。本品溶于PBS(pH7.4)中,蛋白浓度≥1mg/ml,含BSA及甘油等酶稳定剂,可作为标记物用于抗体的制备,黄曲霉毒素及其抗体的免疫检测。经稀释后可用于ELISA检测,效价...
本发明公开了基于HRP催化聚苯胺原位生成用于AFB1的检测方法,包括以下步骤:DNA正四面体探针金电极的合成;将AFB1HRP抗原分别与金电极孵育;然后将孵育后的金电极与苯胺聚合缓冲液反应检测聚苯胺的电化学信号获得标准曲线;将待测样品,AFB1HRP与金电极孵育,然后与苯胺聚合缓冲液反应检测聚苯胺的电化学信号;通过聚苯胺的电化学...
孵育后将电极浸泡在1ml1.66μmedc和1ml4.15μmnhs混合体系中活化2h,加入20μl100ng/ml黄曲霉毒素b1鼠单抗,继续孵育12h后制得sh-t10-cooh探针金电极,像得到的sh-t10-cooh探针金电极表面滴加20μl100ng/mlafb1-hrp抗原和不同浓度黄曲
Our affinity purified mouse anti-AFB1 antibody, conjugated with HRP, can be utilized for the detection and quantization of the mycotoxin aflatoxin AFB1.
The antibody is affinity purified with AFB1-agarose by competitive elution of free AFB1. The antibody is conjugated to horseradish peroxidase (HRP) by reductive amination. This anti-AFB1 HRP conjugates can be utilized for detection and quantification of the food-borne mycotoxin AFB1. Scientific ...