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Virus-based nanoparticles as platform technologies for modern vaccines. Wiley Interdiscip. Rev. Nanomed. Nanobiotechnol. 2016, 8, 554–578. [CrossRef] [PubMed] 84. Zhu, R.; Liu, J.; Chen, C.; Ye, X.; Xu, L.; Wang, W.; Zhao, Q.; Zhu, H.; Cheng, T.; Xia, N. A highly...
However, there is also a heterogeneous availability of solutions; different technologies, protocols, and architectures aim to achieve a seamless interconnection. Henceforth, the standardization still requires great efforts from industrial and scientific environments. In this sense, the interface of the open...
RPMI containing 10% human male AB plasma (Sigma-Aldrich, MO, USA, H4522), 1 mM sodium pyruvate, 2 mM L-glutamine, 0.1 mM MEM nonessential amino acids, 1% penicillin/streptomycin, 10 mM HEPES (Life Technologies, Waltham, MA, USA), 200 IU/mL recombinant human IL-2 (200-02-500UG, ...
While IVM originally began as simple light microscopy, tracking unlabeled cells in thin, translucent tissues [1–4], the technique has continued to evolve, embracing ever-advancing imaging technologies. Two decades ago, IVM utilized single fluorophores and mercury lamp-illuminated fluorescent microscopy ...
Efficiency of transduction was con- firmed by detecting the percentage of GFP-positive cells using Cyan ADP Flow cytometer (Beckman Coulter, Brea, CA, USA) and by Western blot analysis (primary antibody: anti human BRCA1 #9010 Cell Signaling Technologies, Leiden, The Netherlands). Trabectedin (...
Melanocytes were maintained in Medium 254 with Human Melanocyte Growth Supplement-2 (Life Technologies, Carlsbad, CA, USA). To mimic melanoma culture conditions, 10% FBS was added for drug assays. 4.4. ATP Viability Assay, Primary and Secondary Sphere Assays Cell viability was evaluated via a ...
Their results emphasized the combination of different technologies to advance tissue engineering for 'human-on-a-chip' applications [31]. Here, we present a bioprinted tissue equivalent, representing the smallest functional unit of the liver. The printed lobule consists of a hexagonal structure ...
Acknowledgments: The authors acknowledge ScopeM and FIRST laboratories, ETH Zürich for the use of their facilities and continued assistance. In particular we thank Emilio Gini for MOVPE regrowth. Parts of this work have been funded by the Swiss National Foundation under the Nano-tera project "Ir...