When training models such as these we use publicly available train/test splits. However, it should be noted that for the Kather dataset we instead used a randomized 80/20 split on the non-stain-normalized data variant due to the availability of only stain-normalized test data. Non-...
We used 4′,6-diamidino-2-phenylindole (Sigma) to stain the nuclei. Statistical analysis This was a single-arm study, and no randomization was used. To determine the sample size for this clinical trial, overall survival (OS) with standard of care chemotherapy (Gem/Cis) as the historical ...
For quantitative analysis, 10 % cetylpyridinium chloride (CPC; C0732, Sigma-Aldrich) solution was added to each well and incubated for 15 min to elute the stain. The colorimetric measurement was read at a wavelength of 562 nm using a microplate reader (SpectraMax M2, Molecular Devices). 2.8....
SWS is a rare sporadic neurocutaneous syndrome with facial port wine stain involving the first division of the trigeminal nerve, ipsilateral leptomeningeal angioma and angioma involving the ipsilateral eye [1]. This is predominantly a syndrome of pediatric population, rarely presents in adulthood. Altho...
Stain normalization methods for histopathology image analysis: A comprehensive review and experimental comparison. Inf. Fusion 2024, 102, 101997. [Google Scholar] [CrossRef] Nazir, N.; Sarwar, A.; Saini, B.S.; Shams, R. A Robust Deep Learning Approach for Accurate Segmentation of Cytoplasm ...
Thus, diagnosis can be made with IHC using anti-NUT antibodies that stain the nucleus of tumor cells [292,293,294] (Figure 2). The result can be confirmed with a FISH analysis using probes against NUT or even better with NGS to also identify a fusion partner [295,296,297]. Diagnosis ...
Cells were incubated overnight in a humidified chamber with primary antibodies directed toward cell markers specific to the air–blood barrier (Table S4). The primary antibodies were diluted in 2% BSA in PBS. The membranes were washed in PBS and incubated with secondary antibodies conjugated to a...