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FITC-Aβ1-42 peptides were added at 5 μM to MDMi 3D mono- and co-cultures at day 35 of differentiation. After 24 h, cultures were imaged on an EVOS FL Auto 2 (ThermoFisher Scientific, CA, USA). Scans were set to image multiple z-stack planes every 12 h for 7 days using a ...