Taiwan) and Sigma-Aldrich (St. Louis, MO, USA), L929 and RAW 264.7 cells were from ATCC (Manassas, VA, USA). Cell culture media and supplements were obtained from Gibco (Grand Island, NY, USA), including fetal bovine serum (FBS), alpha minimum essential ...
NY, USA). After 24 h incubation at 37 °C, 5% CO2, a cell population enriched in monocytes remained adhered to the culture vessel. Monocytes were then cultured in serum-free RPMI-1640 GlutaMAX medium (Life Technologies, Grand Island, NY, USA) supplemented...
The lines were propagated in Iscove’s medium (IDMEM; GIBCO, Grand Island, NY) supplemented with 20% human plasma and 50 μmol/L 2-mercaptoethanol (2-ME; Sigma, St Louis, MO).34 All lines have retained their clonogenicity in methylcellulose. Growth curves were obtained from liquid suspens...
HK-2 cells were maintained in Dulbecco’s modified Eagle’s medium (DMEM; GIBCO, Grand Island, NY, USA)/Ham’s F-12 Nutrient Mixture medium (F12; GIBCO) at a ratio of 1:1. MMC and MDCK cells were maintained in DMEM. The fresh medium was supplemented with 10% fetal bovine serum (...