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A signal amplification step was then performed by incubation in ABC reagent (Vector Laboratories, PK-6100, Vectastain Elite ABC-Peroxidase Kits; 1:1000; for 1 h) followed by a reaction with 0.05% 3,3-diaminobenzidine tetrahydrochloride/0.01% hydrogen peroxide (Sigma, D5637-5G) in PBS. ...
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For each of the 48 adult hens, DNA was extracted from a 200 mg section of intestinal tissue and caecal content using ZymoBIOMICS DNA minikits (Cambridge Bioscience, UK) according to the manufacturer’s instructions. The section was cut using a sterile scalpel blade to expose the mucosa and ...
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