After treating with avidin–biotin blocking solution, the cytospin smears were stained with primary antibody and, after overnight incubation at 25 1C, biotinylated secondary antibody in a 1 : 200 dilution in 5% BSA was applied. Visualization was carried out with streptavidin–-FITC (1 : 1000)....
containing 20 ml of Additive-A (BSA-20; TCS, Scientific Corp.) and 10 ml of anti-foaming agent (AFA-25; TCS, Scientific Corp.). The samples were oxygenated with air supplied from an external tank and deoxygenated with nitrogen gas. Changes in oxygen tension were measured using a Clark ...
2D = 2 dimensional; BSA = body surface area; CWD = continuous-wave Doppler; LA = left atrium; LV = left ventricle; MM = M-mode; MV = mitral valve; PW = pulsed wave; RV = right ventricle; RVD = right ventricular diameter; TAPSE = tricuspid annular plane systolic excursion; TDI = ...
BSA, Body surface area; IGA, Investigator Global Assessment; ITT, intent-to-treat; WI-NRS, Worst Itch Numeric Rating Scale. ∗ Determined by the patient's hand method, where the patient's hand (including fingers) surface area was assumed to equal 1% of BSA. † This global assessment ...
Antibody anti-ERK 2 (rabbit) Santa Cruz Biotecnology sc-154 Dilution 1:1,000 in BSA 2.5% Antibody anti-pERK (mouse) Santa Cruz Biotecnology sc-7383 Dilution 1:1,000 in BSA 2.5% B27 Gibco 17504-044 BDNF Sigma Aldrich SRP3014 Bovine Serum Albumin Sigma Aldrich A7906 Bra...
The membranes were then blocked with 5% BSA, incubated in turn with primary and IRDye®-conjugated secondary antibodies (1:500 – 1:2000 dilutions), in turn. Blots or dots were visualized using an Odyssey Imaging System (LI-COR Biosciences), and quantified using Quantity One software (Bio...
This BSA-AAL composite atlas was resampled in the 2 Â 2 Â 2 mm3 grid MNI space. The representative time course in each region was extracted by averaging the time courses of the voxels therein. A band-pass filter (transmission range, 0.008–0.1 Hz) was applied to these sets of time...
The wells were aspirated free of fluid and washed three times with PBS + 0.05% Tween-20, and 100 µL of goat anti-mouse IgM (µ-specific) peroxidase (Sigma) diluted 1:4,000 in PBS + 0.25% BSA was added for 1 hour at room temperature. The wells were aspirated free of fluid ...
It is preferred to use 10% human serum with all growth factors except IGF-I, IGF-II or PDGF with which a supplement of 1% human serum, transferrin and bovine serum albumin (BSA) is preferred. The growth medium is changed at suitable intervals, preferably at about 3 days of incubation. ...
Between steps, slides were washed with PBS/BSA. Cells were examined by fluorescence microscopy (Zeiss Axiovert; Carl Zeiss, Inc., Thornwood, N.Y.) and confocal fluorescence microscopy (Confocal 1024 microscope; Olympus AX70, Olympus Optical Co. LTD, Japan). To assess the frequency of ...