Poly(ADP-ribose) is a nucleic acid-like homopolymer composed of repeating adenosine diphosphate ribose units (Fig. 1.1). The poly(ADP-ribose) polymer found in eukaryotic cells exhibits variable chain length, ranging from two to more than 200 ADP-ribosyl residues (Hayashi et al. 1983; Alvarez-...
2022年9月30日,澳大利亚昆士兰大学Bostjan Kobe团队和格里菲斯大学Thomas Ve团队联合在国际综合学术期刊Science上在线发表了题为“Cyclic ADP ribose isomers: Production, chemical structures, and immune signaling”的研究论文,该研究鉴定了cADPR异构体的化学结构和环化位点、由细菌TIR结构域催化产生cADPR异构体的分子结构...
近日，澳大利亚昆士兰大学Bostjan Kobe与格林菲斯大学的Thomas Ve联合领导的研究团队在Science发表研究长文Cyclic ADP ribose isomers: Production, chemical structures, and immune signaling，系统介绍cADPR异构体的环化位点、由TIR结构域催化产生的分子机制、及其在细菌和植物免疫通路中的功能。作者首先表达并纯化出鲍曼不动...
近日,澳大利亚昆士兰大学Bostjan Kobe与格林菲斯大学的Thomas Ve联合领导的研究团队在Science发表研究长文Cyclic ADP ribose isomers: Production, chemical structures, and immune signaling,系统介绍cADPR异构体的环化位点、由TIR结构域催化产生的分子机制、及其在细菌和植物...
The structure of cADPR is unique. The long linear molecule of NAD is completely circularized, with the adenine group linked back to the distal ribose unit in a head-to-tail manner (Fig. 7.1). The sites of linkage are the N1-nitrogen of the adenine ring and the anomeric carbon of the ...
Miwa M, Sugimura T (1984) Structure of poly(ADP-ribose). Methods Enzymol 106:441–450Miwa M, and Sugimura T. (1984) Structure of poly(ADP-ribose). Methods Enzymol 25 106:441-450. 26 5. Berger NA. (1985) Poly(ADP-ribose) in the cellular response to DNA damage. Radiat 27 Res 101...
近日,澳大利亚昆士兰大学Bostjan Kobe与格林菲斯大学的Thomas Ve联合领导的研究团队在Science发表研究长文Cyclic ADP ribose isomers: Production, chemical structures, and immune signaling,系统介绍cADPR异构体的环化位点、由TIR结构域催化产生的分子机制、及其在细菌和植物免疫通路中的功能。
二磷酸腺苷核糖基化(ADP-ribosylation)是一种蛋白翻译后修饰,利用NAD+作为供体,PARPs(Poly ADP-ribose polymerases)作为合成酶将ADP-ribose连接到目的蛋白。与其他蛋白翻译后修饰(甲基化,磷酸化等)类似的是,二磷酸腺苷核糖基化也是可逆的过程,ADP-ribose可...
Poly(ADP-ribose) polymerase (PARP) participates in the intricate network of systems developed by the eukaryotic cell to cope with the numerous environmental and endogenous genetoxic agents. Cloning of the PARP gene has allowed the development of genetic and molecular approaches to elucidate the struc...
Here we describe the chemical synthesis of the ADP-ribose dimer, and we use this compound to obtain the first human PARG substrate-enzyme cocrystal structure. Chemical synthesis of PAR is an attractive alternative to traditional enzymatic synthesis and fractionation, allowing access to products such ...