ADP emphasized that the fraudsters needed to have the victim’s personal data — including name, date of birth and Social Security number — to successfully create an account in someone’s name. ADP also stressed that this personal data did not come from its systems, and that thieves appeared...
The membranes were suspended to 0.2 mg/ml in a buffer containing 1 mM Tricine, 50 mM KCl, 2.0 mM MgCl2, NaOH to pH 8.0 (adjusted immediately prior to each measurements), 1 μM valinomycin to abolish Δφ, and 100μM Phenol Red. The pH changes of the suspension were followed as a ...
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These results indicated that TbMCP5 was about 4-fold less abundant than ScAnc2p in the isolated mitochondrial fractions, which in turn could account for the reduced growth of JL-TbMCP5 on YPL medium. ADP/ATP Transport Kinetics of TbMCP5 in Comparison with ScAnc2p The ADP/ATP exchange ...
Traditional biochemical studies regarding functional specificity in extant homologous enzymes often focus on the identification of key amino acid changes that would account for different functions in different families (8). However, during evolution most changes are expected to have a small or null effec...
41 These additional interactions may also account for the apparent increased ordering of the D loop in yeast actin (Figure S1B). The D loop is susceptible to proteolytic cleavage by subtilisin between Met47 and Gly48 of the D loop (Figures S1B and S1E).23 The rate of subtilisin digestion...
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After growth at 16°C for 12 h, the cells were harvested, re-suspended in lysis buffer (1 × PBS, 2 mM DTT and 1 mM PMSF) and lysed by sonication. The cell lysate was centrifuged at 20,000 g for 45 min at 4°C to remove cell debris. The supernatant was applied onto a self-...