To compare microarrray experiments performed in obese and lean subjects, we used a common reference pool generated by mixing equal amounts of total RNA extracted from adipose tissue samples of all analyzed patients. aRNA from the reference pool was labeled with Cy3, while the aRNA from the test...
times in 0.01 M PBS, for 5 min each time, and were then mounted with an antifade solution. Labeled cells were examined under a fluorescence microscope (Axiphot; Zeiss, Oberkochen, Baden-Wurttemberg, Germany). Image-Pro Plus (Media Cybernetics, USA) was used to acquire and process t...
(IML) cell column of the spinal cord shows a separate control of the compartments; therefore, the survival time of the animals was chosen so that either only second-order or third-order neurons were labeled. (B) In an upstream direction, the PVN of the hypothalamus shows specialized sets ...
Total RNA was collected at slaughter from outer subcutaneous adipose tissue (OSQ) and middle subcutaneous adipose tissue (MSQ) from gilts at 90, 150, and 210d (=5age). Dye labeled cDNA probes were hybridized to custom porcine microarrays (70-mer oligonucleotides). Gene expression of insulin-...
17, 34 Thus, nEVs and oEVs that are labeled with PKH26 dye were evaluated for incorporation into target cells. nEVs and oEVs were incorporated by ECs as shown in Figure 2c. Moreover, we show that this internalization depends on the presence of the CD29 surface marker (Figure 2c). ...
Drs Allen and Hines Jr from the Mayo clinic labeled this condition as lipedema in 1940[111]. Outside the US, lipedema is known as "lipoedema", meaning edema of the fat. This disorder is likely very common but underdiagnosed. Diagnosis The diagnosis of lipedema is made clinically by ...
Untreated hAd-MSCs at passage 3 were treated with 0.25% trypsin-EDTA, extracted, and washed twice with DPBS. The cells were subsequently incubated on ice and labeled with mouse anti-human antibodies for FITC-CD45 (Cat. Num: 982316), PE-CD34 (Cat. Num: 343504), PE-CD73 (Cat. Num: 34...
3a). Cell tracking in the ROI of the distal section identified Dil-labeled cells within the augmented muscular tissue (Fig. 4c, d). Those cells were located adjacent to the sites displaying α-SMA and RhoA signals. However, α-SMA and RhoA were not expressed at the same sites, and they...
To fully elucidate on the biodistribution and biodegradation of rGO, the material should be labeled and scaffolds should be evaluated at both early (days to weeks) and later experimental timepoints (months to a year), but this was beyond the scope of the current study. Although formation of ...
Human CD271+ or CD271− progenitors were labeled by PKH as described in cell therapy method section and co-cultured with HUVEC in 1:2 ratio (13,000 cells). Co-culture samples and negative control samples were maintained by M199 media without cocktail of angiogenic growth factor supplements....