doi:US6730827 B1Saverio Carl FalcoUS
The light pulse is generated in a two step reaction where the released pyrophosphate reacts with ATP sulfurylase and adenosine phosphosulfate to form ATP, and then ATP and luciferase oxidize luciferin to release the light. This method of sequencing typically is used for shorter pieces of DNA or ...
Adenosine 5{prime}-phosphosulfate kinase (APSK) catalyzes the ATP-dependent synthesis of adenosine 3{prime}-phosphate 5{prime}-phosphosulfate (PAPS), which is an essential metabolite for sulfur assimilation in prokaryotes and eukaryotes. Using APSK from Arabidopsis thaliana, we examine the energetics ...