Schuster S, Kenanov D (2005) Adenine and adenosine salvage pathways in erythrocytes and the role of S-adenosylhomo- cysteine hydrolase: a theoretical study using elementary flux modes. FEBS J 272:5278-5290Schuster, S. und D. Kenanov (2005). "Adenine and adenosine salvage pathways in ...
After 5 min on ice, soluble (supernatant of 6800 g) and insoluble (pellet of 20,000 g) fractions were separated by centrifugation. 2.5. Purine transport assays Uptake of [3H]-hypoxanthine and [3H]-adenosine (both from GE Healthcare) was performed essentially as described previously (de ...
The measurement of adenosine and adenosine-related compounds is of interest to workers in many different fields. As the purpose of research varies so do the compounds of interest, the sensitivity required of the assay, and the number of possible pitfalls in the difficult task of producing a meas...
2.5Nicotinamide adenine dinucleotide (NAD+) and cardiac aging Nicotinamide adenine dinucleotide(NAD+) is an electron acceptor in the mitochondrialelectron transport chain. Cellular NAD+levels are regulated by the balance between the synthesis through de novo and salvage pathways and the consumption bysirt...
This autosomal recessive trait results in inability to salvage adenine, which accumulates and is oxidized to 2,8-dihydroxyadenine by xanthine oxidase. The main clinical abnormality is the excretion of 2,8-dihydroxyadenine as insoluble material (gravel) in the urine. These stones can be confused ...
(AMP) in yeast and adenosine diphosphate (ADP) in mammals, and inhibition by adenosine triphosphate (ATP), are properties associated with sensitive regulation of respiratory rates by cellular energy charge(23). More recently, an additional possible role in direct regulation of expression has emerge...
4.1.2 Emerging substrates for adenosine production in the BM microenvironment 4.1.2.1 NAD+ as the alternative salvage substrate While the expression of CD38 by human mPCs is known since long [105,106], its role in the production of ADO is however less studied. Our Laboratory confirmed that...
SAC1 generates cyclic adenosine monophosphate (cAMP), which itself has a melanin-dependent inhibitory effect on spore germination (Dirschnabel et al., 2014; Kamerewerd et al., 2008). Thus, cAMP seems to mimic the ascospore germination defect of the nox2, nor1, and ste12 deletion mu...
Reactions were started by adding 20 mg/ml luciferase (Roche) and Luciferin (Sigma) (0.8 mM in 0.5 M Tris–acetate, pH 7.75) to this buffer, also containing 20 μM di-(adenosine pentaphosphate) (AP5A) to inhibit cytosolic adenylate kinase. Luminescence was continuously recorded over 2 s, ...
2. Measurements and sources of extracellular adenine compounds Once it became clear that adenine compounds (Ap3A, Ap4A, ApnA, ATP, ADP, AMP, adenosine and cAMP) were physiological components within the cardiovascular system it was important to develop accurate methods to measure the plasma content...