The CCA-adding enzyme [ATP(CTP):tRNA nucleotidyltransferase] builds and repairs the 3' terminal CCA sequence of all tRNAs by adding one nucleotide at a time, using CTP and ATP as substrates. Unlike all other sequence-specific RNA and DNA polymerases, the CCA-adding enzyme does not use a...
The first detectable U2 snRNA precursor contains 10–16 extra 3′ nucleotides that are removed by one or more 3′ exonucleases. Thus, if 3′ exonuclease activity removes the encoded 3′ CC during U2 snRNA maturation, as appears to be the case in vitro, the cell may need to build or ...
S3B). A fascinating possibility currently being explored is that helix L may break or partially dislocate at Pro-295 (or the equivalent glycine in homologous enzymes) during one or more steps in CCA addition. Modeling the C74 Addition Complex To explain CCA addition without tRNA translocation, ...
Accordingly, it is an object of the present invention to overcome one or more drawbacks or disadvantages of the prior art by providing a functional food or a nutritional supplement containing an activated folate that is readily absorbed in the intestines of a human, and which is reduced by ...
The CCA-adding enzyme [ATP(CTP):tRNA nucleotidyltransferase] builds and repairs the 3' terminal CCA sequence of all tRNAs by adding one nucleotide at a time. Unlike all other sequence-specific RNA and DNA polymerases, the CCA-adding enzyme does not use a nucleic acid template. Thus the pr...
or powder activated carbon (PAC) 135 is added prior to introduction into a contactor basin 150 within the detention tank battery 100 and finally sent to a filter battery 200 having at least one filter column at one or more levels to remove any contaminants or by-products from the water ...
are known to produce more flexible enzymes in order to lower their activation energy [2] and the central question for CCA-adding enzymes is how this adaptation can be achieved without compromising the specificity of the reaction, which requires an accurate selection of incorporated nucleotides. To...
Yeast Vps proteins have one or more human orthologues, indicating a conserved association with the ESCRT pathway in mammals. Importantly, the CHMP2B gene is one of the two human orthologues of the yeast Vps2 protein, which is encoded to form the components of the heteromeric ESCRT-III complex...