获取 NGS 的方法有多种,但最常用的方法之一是 Illumina NGS 平台。这是 Addgene 的测序合作伙伴 seqWell(https://blog.addgene.org/the-power-behind-ngs-plasmid-validation-seqwell?_ga=2.224584666.1761310573.1650342339-1554568351.1648354532)使用的平台。 Illumina NGS 的实际过程与 Sanger 测序没有太大区别。该...
sequencing_primer_3 sequencing_primer_5 vector_types Mammalian Expression AAV growth notes : This plasmid contains the mouse calcium/calmodulin-dependent kinase II alpha subunit promoter. For additional information please visit - http://www.optogenetics.org ...
(Zuo et al., 2007). Addgene was started the same year as a nonprofit organization and was initially funded by private donations and small business loans (Fan et al., 2005). Both platforms are backed up by solid plasmid storage and sequencing facilities and distribute their samples ...
plasmid copy : myristoylated Akt1 gene from P. Tsichlis resistance markers : 52 tags : Unknown terms : Neomycin (select with G418) more info or order: Addgene product webpage company information Addgene 490 Arsenal Way, Suite 100 Watertown, MA 02472 ...
Pricing$350 USD for preparation of 100 µL virus + $30 USD for plasmid. StorageStore at -80℃. Thaw just before use and keep on ice. ShipmentViral particles are shipped frozen on dry ice. Plasmid DNA (≥ 200ng) will also be included in the shipment. ...
4、 (关于 Addgene)三查找方法1.我们以常见的 pRS质粒为列,点击菜单栏的Plasmid Referenee ,进入以下界面L旳in | New U理Oaddge neA better way ta snare plasmidsHomsAbout AddgenePlasmid Guide0e忖 ovaigm3D $曲:嬉皿:亡s别广劭09亡11亡iiOHitQ0ertBacterial ResistanceMpKJlIin 畑nainyfincniiwarnDhEr...
Addgene PlasmidDescription pLKO.1 – TRC control Negative control vector containing non-hairpin insert. pLKO.1 – scramble shRNA Negative control vector containing scrambled shRNA. psPAX2 Packaging plasmid for producing viral particles. pMD2.G Envelope plasmid for producing viral particles. Note...
For transient knockdown of protein expression, you may transfect plasmid DNA directly into the target cells. The shRNA will be expressed, but the DNA is unlikely to be integrated into the host genome. For stable loss-of-function experiments, Addgene recommends that you generate lentiviral particle...
2014年8月10日Addgene - pMD2.G Plasmid Data https://www.addgene.org/12259/1/3 Purpose:Gene/insert name:Vector backbone:Vector type:Backbone size w/o insert (bp):5' sequencing primer:Bacterial resistance(s)Growth strain(s)Growth temperature (℃):High or low copy:Sequence:Map:Supplemental ...
as a leader in r&d genomics services, genewiz provides superior data and high-quality constructs for next generation sequencing, gene さらに表示する グローバルランク #167,969addgene.org addgene, the nonprofit plasmid repository, accelerates science by sharing useful plasmid-based research materials...