The potential for generation of replication-competent lentivirus is addressed by the design of the vectors and by safe laboratory practice. In terms of vector design, 2nd and 3rd generation lentiviral systems provided by Addgene separate transfer, envelope, and packaging components of the virus onto ...
Self-inactivating lentivirus vector for safe and efficient in vivo gene delivery. . Zufferey R et. al. J Virol . 1998. 72(12):9873-80.PubMed. H.2. Web resources Addgene’s mammalian RNAi website:www.addgene.org/mammalianrnai The RNAi Consortium (TRC):www.broad.mit.edu/genome_bio/...
Requestor is responsible for compliance withtheir institution"s biosafety regulations.Lentivirus is generally considered BSL-2. AAV isgenerally considered BSL-1, but may requireBSL-2 handling depending on the insert.Biosafety Guide Resource Information ...
Lentivirus-delivered stable gene silencing by RNAi in primary cells.Stewart SA et. al.RNA. 2003. 9(4):493-501.PubMed. Multiply attenuated lentiviral vector achieves efficient gene delivery in vivo.Zufferey R et. al.Nat Biotechnol. 1997. 15(9):871-5.PubMed. Self-inactivating lentivirus vecto...